<i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina

Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage trea...

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Autores principales: Rosso, Lorena Beatriz, Sedan, Daniela Yazmine, Kolman, María de los Ángeles, Caixach, Josep, Flores, Cintia, Oteiza, Juan Manuel, Salerno, Graciela Lidia, Echenique, Ricardo Omar, Giannuzzi, Leda, Andrinolo, Darío
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97702
https://ri.conicet.gov.ar/11336/10936
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97702
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
spellingShingle Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
Rosso, Lorena Beatriz
Sedan, Daniela Yazmine
Kolman, María de los Ángeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Manuel
Salerno, Graciela Lidia
Echenique, Ricardo Omar
Giannuzzi, Leda
Andrinolo, Darío
<i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
topic_facet Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
description Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
format Articulo
Articulo
author Rosso, Lorena Beatriz
Sedan, Daniela Yazmine
Kolman, María de los Ángeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Manuel
Salerno, Graciela Lidia
Echenique, Ricardo Omar
Giannuzzi, Leda
Andrinolo, Darío
author_facet Rosso, Lorena Beatriz
Sedan, Daniela Yazmine
Kolman, María de los Ángeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Manuel
Salerno, Graciela Lidia
Echenique, Ricardo Omar
Giannuzzi, Leda
Andrinolo, Darío
author_sort Rosso, Lorena Beatriz
title <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_short <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_full <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_fullStr <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_full_unstemmed <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_sort <i>microcystis aeruginos</i> strain [d-leu1] mcyst-lr producer, from buenos aires province, argentina
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/97702
https://ri.conicet.gov.ar/11336/10936
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