Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling

Background: In HIV-1 infected cells, the integrated viral DNA is transcribed by the host cell machinery to generate the full length HIV-1 RNA (FL RNA) that serves as mRNA encoding for the Gag and GagPol precursors. Virion formation is orchestrated by Gag, and the current view is that a specific inte...

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Autores principales: El Mekdad, Hala, Boutant, Emmanuel, Karnib, Hassan, Biedma, Marina Elizabeth, Sharma, Kamal Kant, Malytska, Iuliia, Laumond, Géraldine, Roy, Marion, Réal, Eléonore, Paillart, Jean Christophe, Moog, Christiane, Darlix, Jean Luc, Mély, Yves, de Rocquigny, Hugues
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Biología
HIV
Gag
RPL7
Interaction
Chaperone activity
Nucleocapsid
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97566
https://ri.conicet.gov.ar/11336/57847
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982112/
https://retrovirology.biomedcentral.com/articles/10.1186/s12977-016-0287-4
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97566
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
HIV
Gag
RPL7
Interaction
Chaperone activity
Nucleocapsid
spellingShingle Biología
HIV
Gag
RPL7
Interaction
Chaperone activity
Nucleocapsid
El Mekdad, Hala
Boutant, Emmanuel
Karnib, Hassan
Biedma, Marina Elizabeth
Sharma, Kamal Kant
Malytska, Iuliia
Laumond, Géraldine
Roy, Marion
Réal, Eléonore
Paillart, Jean Christophe
Moog, Christiane
Darlix, Jean Luc
Mély, Yves
de Rocquigny, Hugues
Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling
topic_facet Biología
HIV
Gag
RPL7
Interaction
Chaperone activity
Nucleocapsid
description Background: In HIV-1 infected cells, the integrated viral DNA is transcribed by the host cell machinery to generate the full length HIV-1 RNA (FL RNA) that serves as mRNA encoding for the Gag and GagPol precursors. Virion formation is orchestrated by Gag, and the current view is that a specific interaction between newly made Gag molecules and FL RNA initiates the process. This in turn would cause FL RNA dimerization by the NC domain of Gag (GagNC). However the RNA chaperoning activity of unprocessed Gag is low as compared to the mature NC protein. This prompted us to search for GagNC co-factors. Results: Here we report that RPL7, a major ribosomal protein involved in translation regulation, is a partner of Gag via its interaction with the NC domain. This interaction is mediated by the NC zinc fingers and the N- and C-termini of RPL7, respectively, but seems independent of RNA binding, Gag oligomerization and its interaction with the plasma membrane. Interestingly, RPL7 is shown for the first time to exhibit a potent DNA/RNA chaperone activity higher than that of Gag. In addition, Gag and RPL7 can function in concert to drive rapid nucleic acid hybridization. Conclusions: Our results show that GagNC interacts with the ribosomal protein RPL7 endowed with nucleic acid chaperone activity, favoring the notion that RPL7 could be a Gag helper chaperoning factor possibly contributing to the start of Gag assembly.
format Articulo
Articulo
author El Mekdad, Hala
Boutant, Emmanuel
Karnib, Hassan
Biedma, Marina Elizabeth
Sharma, Kamal Kant
Malytska, Iuliia
Laumond, Géraldine
Roy, Marion
Réal, Eléonore
Paillart, Jean Christophe
Moog, Christiane
Darlix, Jean Luc
Mély, Yves
de Rocquigny, Hugues
author_facet El Mekdad, Hala
Boutant, Emmanuel
Karnib, Hassan
Biedma, Marina Elizabeth
Sharma, Kamal Kant
Malytska, Iuliia
Laumond, Géraldine
Roy, Marion
Réal, Eléonore
Paillart, Jean Christophe
Moog, Christiane
Darlix, Jean Luc
Mély, Yves
de Rocquigny, Hugues
author_sort El Mekdad, Hala
title Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling
title_short Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling
title_full Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling
title_fullStr Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling
title_full_unstemmed Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling
title_sort characterization of the interaction between the hiv-1 gag structural polyprotein and the cellular ribosomal protein l7 and its implication in viral nucleic acid remodeling
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/97566
https://ri.conicet.gov.ar/11336/57847
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982112/
https://retrovirology.biomedcentral.com/articles/10.1186/s12977-016-0287-4
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