Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production

Bradyrhizobium diazoefficiens, a soybean N2-fixing symbiont, constitutes the basic input in one of the most prominent inoculant industries worldwide. This bacterium may be cultured with D-mannitol or L-arabinose as carbon-plus-energy source (C-source) with similar specific growth rates, but with hig...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Cogo, Carolina, Pérez Giménez, Julieta, Rajeswari, Chandrasekar B., Luna, María Flavia, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Bradyrhizobium
CBB pathway
Flagella
L-KDA pathway
O2 consumption
PP pathway
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97514
https://ri.conicet.gov.ar/11336/96974
https://www.frontiersin.org/articles/10.3389/fmicb.2018.01189/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996035/
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97514
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bradyrhizobium
CBB pathway
Flagella
L-KDA pathway
O2 consumption
PP pathway
spellingShingle Biología
Bradyrhizobium
CBB pathway
Flagella
L-KDA pathway
O2 consumption
PP pathway
Cogo, Carolina
Pérez Giménez, Julieta
Rajeswari, Chandrasekar B.
Luna, María Flavia
Lodeiro, Aníbal Roberto
Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production
topic_facet Biología
Bradyrhizobium
CBB pathway
Flagella
L-KDA pathway
O2 consumption
PP pathway
description Bradyrhizobium diazoefficiens, a soybean N2-fixing symbiont, constitutes the basic input in one of the most prominent inoculant industries worldwide. This bacterium may be cultured with D-mannitol or L-arabinose as carbon-plus-energy source (C-source) with similar specific growth rates, but with higher biomass production with D-mannitol. To better understand the bacterium's carbon metabolism, we analyzed, by liquid chromatography and tandem mass spectrometry (MS), the whole set of proteins obtained from cells grown on each C-source. Among 3,334 proteins identified, 266 were overproduced in D-mannitol and 237 in L-arabinose, but among these, only 22% from D-mannitol cultures and 35% from L-arabinose cultures were annotated with well defined functions. In the D-mannitol-differential pool we found 19 enzymes of the pentose-phosphate and Calvin-Benson-Bassham pathways and accordingly observed increased extracellular-polysaccharide production by D-mannitol grown bacteria in a CO2-enriched atmosphere. Moreover, poly-3-hydroxybutyrate biosynthesis was increased, suggesting a surplus of reducing power. In contrast, the L-arabinose-differential pool contained 11 enzymes of the L-2-keto-3-deoxyarabonate pathway, 4 enzymes for the synthesis of nicotinamide-adenine dinucleotide from aspartate, with those cultures having a threefold higher O2-consumption rate than the D-mannitol cultures. The stoichiometric balances deduced from the modeled pathways, however, resulted in similar O2 consumptions and ATP productions per C-mole of substrate. These results suggested higher maintenance-energy demands in L-arabinose, which energy may be used partly for flagella-driven motility. Since B. diazoefficiens produces the lateral-flagella system in only L-arabinose, we calculated the O2-consumption rates of a lafR::Km mutant devoid of lateral flagella cultured in L-arabinose or D-mannitol. Contrary to that of the wild-type, the O2-consumption rate of this mutant was similar on both C-sources, and accordingly outcompeted the wild-type in coculture, suggesting that the lateral flagella behaved as parasitic structures under these conditions. Proteomic data are available via ProteomeXchange with identifier PXD008263.
format Articulo
Articulo
author Cogo, Carolina
Pérez Giménez, Julieta
Rajeswari, Chandrasekar B.
Luna, María Flavia
Lodeiro, Aníbal Roberto
author_facet Cogo, Carolina
Pérez Giménez, Julieta
Rajeswari, Chandrasekar B.
Luna, María Flavia
Lodeiro, Aníbal Roberto
author_sort Cogo, Carolina
title Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production
title_short Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production
title_full Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production
title_fullStr Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production
title_full_unstemmed Induction by Bradyrhizobium diazoefficiens of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO2 Fixation, O2 Consumption, and Lateral-Flagellum Production
title_sort induction by bradyrhizobium diazoefficiens of different pathways for growth in d-mannitol or l-arabinose leading to pronounced differences in co2 fixation, o2 consumption, and lateral-flagellum production
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97514
https://ri.conicet.gov.ar/11336/96974
https://www.frontiersin.org/articles/10.3389/fmicb.2018.01189/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996035/
work_keys_str_mv AT cogocarolina inductionbybradyrhizobiumdiazoefficiensofdifferentpathwaysforgrowthindmannitolorlarabinoseleadingtopronounceddifferencesinco2fixationo2consumptionandlateralflagellumproduction
AT perezgimenezjulieta inductionbybradyrhizobiumdiazoefficiensofdifferentpathwaysforgrowthindmannitolorlarabinoseleadingtopronounceddifferencesinco2fixationo2consumptionandlateralflagellumproduction
AT rajeswarichandrasekarb inductionbybradyrhizobiumdiazoefficiensofdifferentpathwaysforgrowthindmannitolorlarabinoseleadingtopronounceddifferencesinco2fixationo2consumptionandlateralflagellumproduction
AT lunamariaflavia inductionbybradyrhizobiumdiazoefficiensofdifferentpathwaysforgrowthindmannitolorlarabinoseleadingtopronounceddifferencesinco2fixationo2consumptionandlateralflagellumproduction
AT lodeiroanibalroberto inductionbybradyrhizobiumdiazoefficiensofdifferentpathwaysforgrowthindmannitolorlarabinoseleadingtopronounceddifferencesinco2fixationo2consumptionandlateralflagellumproduction
bdutipo_str Repositorios
_version_ 1764820492862095360

Ejemplares similares