Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations

Background: Bovine leukocyte antigens (BoLAs) are used extensively as markers of disease and immunological traits in cattle. However, until now, characterization of BoLA gene polymorphisms in Zebu breeds using high resolution typing methods has been poor. Here, we used a polymerase chain reaction se...

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Detalles Bibliográficos
Autores principales: Takeshima, Shin nosuke, Corbi Botto, Claudia, Giovambattista, Guillermo, Aida, Yoko
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
BoLA-DRB3
Brahman
Genetic diversity
Gir
Nellore
Sequence-based typing
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97487
https://ri.conicet.gov.ar/11336/82791
https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-018-0618-7
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97487
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
BoLA-DRB3
Brahman
Genetic diversity
Gir
Nellore
Sequence-based typing
spellingShingle Ciencias Veterinarias
BoLA-DRB3
Brahman
Genetic diversity
Gir
Nellore
Sequence-based typing
Takeshima, Shin nosuke
Corbi Botto, Claudia
Giovambattista, Guillermo
Aida, Yoko
Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations
topic_facet Ciencias Veterinarias
BoLA-DRB3
Brahman
Genetic diversity
Gir
Nellore
Sequence-based typing
description Background: Bovine leukocyte antigens (BoLAs) are used extensively as markers of disease and immunological traits in cattle. However, until now, characterization of BoLA gene polymorphisms in Zebu breeds using high resolution typing methods has been poor. Here, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 421 cattle (116 Bolivian Nellore, 110 Bolivian Gir, and 195 Peruvian Nellore-Brahman). Data from 1416 Taurine and Zebu samples were also included in the analysis. Results: We identified 46 previously reported alleles and no novel variants. Of note, 1/3 of the alleles were detected only in Zebu cattle. Comparison of the degree of genetic variability at the population and sequence levels with genetic distance in the three above mentioned breeds and nine previously reported breeds revealed that Zebu breeds had a gene diversity score higher than 0.86, a nucleotide diversity score higher than 0.06, and a mean number of pairwise differences greater than 16, being similar to those estimated for other cattle breeds. A neutrality test revealed that only Nellore-Brahman cattle showed the even gene frequency distribution expected under a balanced selection scenario. The FST index and the exact G test showed significant differences across all cattle populations (FST =0.057; p < 0.001). Neighbor-joining trees and principal component analysis identified two major clusters: one comprising mainly European Taurine breeds and a second comprising Zebu breeds. This is consistent with the historical and geographical origin of these breeds. Some of these differences may be explained by variation of amino acid motifs at antigen-binding sites. Conclusions: The results presented herein show that the historical divergence between Taurine and Zebu cattle breeds is a result of origin, selection, and adaptation events, which would explain the observed differences in BoLA-DRB3 gene diversity between the two major bovine types. This allelic information will be important for investigating the relationship between the major histocompatibility complex and disease, and contribute to an ongoing effort to catalog bovine MHC allele frequencies according to breed and location.
format Articulo
Articulo
author Takeshima, Shin nosuke
Corbi Botto, Claudia
Giovambattista, Guillermo
Aida, Yoko
author_facet Takeshima, Shin nosuke
Corbi Botto, Claudia
Giovambattista, Guillermo
Aida, Yoko
author_sort Takeshima, Shin nosuke
title Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations
title_short Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations
title_full Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations
title_fullStr Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations
title_full_unstemmed Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations
title_sort genetic diversity of bola-drb3 in south american zebu cattle populations
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97487
https://ri.conicet.gov.ar/11336/82791
https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-018-0618-7
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AT giovambattistaguillermo geneticdiversityofboladrb3insouthamericanzebucattlepopulations
AT aidayoko geneticdiversityofboladrb3insouthamericanzebucattlepopulations
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