Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis

A novel arginine-based cationic surfactant N<sup>α</sup>-benzoyl-arginine dodecylamide (Bz-Arg-NHC<sub>12</sub>) was synthesized in our laboratory. In this paper we study the interaction of Bz-Arg-NHC<sub>12</sub> with sheep and human red blood cells (SRBC and HRB...

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Autores principales: Fait, María Elisa, Hermet, Melisa, Vázquez, Romina Florencia, Maté, Sabina María, Daza Millone, María Antonieta, Vela, María Elena, Morcelle del Valle, Susana Raquel, Bakás, Laura Susana
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Física
Biología
Amino acid-based surfactants
Antihemolytic potency
Hemolysis
Membrane physicochemical properties
Microvesicles
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97454
https://ri.conicet.gov.ar/11336/83132
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97454
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Física
Biología
Amino acid-based surfactants
Antihemolytic potency
Hemolysis
Membrane physicochemical properties
Microvesicles
spellingShingle Física
Biología
Amino acid-based surfactants
Antihemolytic potency
Hemolysis
Membrane physicochemical properties
Microvesicles
Fait, María Elisa
Hermet, Melisa
Vázquez, Romina Florencia
Maté, Sabina María
Daza Millone, María Antonieta
Vela, María Elena
Morcelle del Valle, Susana Raquel
Bakás, Laura Susana
Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
topic_facet Física
Biología
Amino acid-based surfactants
Antihemolytic potency
Hemolysis
Membrane physicochemical properties
Microvesicles
description A novel arginine-based cationic surfactant N<sup>α</sup>-benzoyl-arginine dodecylamide (Bz-Arg-NHC<sub>12</sub>) was synthesized in our laboratory. In this paper we study the interaction of Bz-Arg-NHC<sub>12</sub> with sheep and human red blood cells (SRBC and HRBC respectively) due to their different membrane physicochemical/biophysical properties. SRBC demonstrated to be slightly more resistant than HRBC to the hemolytic effect of the surfactant, being the micellar structure responsible for the hemolytic effect in both cases. Moreover, besides the hemolytic effect, a dual behavior was observed for the surfactant studied: Bz-Arg-NHC<sub>12</sub> was also able to protect red blood cells against hypotonic lysis for HRBC in a wide range of surfactant concentrations. However, the degree of protection showed for SRBC was about 50% lower than for HBRC. In this regard, a remarkable volume expansion was evidenced only for SRBC treated with Bz-Arg-NHC<sub>12</sub>, although no correlation with the antihemolytic potency (pAH) was found. On the contrary, our surfactant showed a greater pAH when human erythrocytes were submitted to hypotonic stress, with a low volume expansion, showing a higher amount of solubilized phospholipids in the supernatant when compared with SRBC behavior. Surface plasmon resonance measurements show the molecular interaction of the surfactant with lipid bilayers from HRBC and SRBC lipids, demonstrating that in the latter neither microvesicle release or lipid extraction occurred. Our results demonstrate that the volume expansion of erythrocytes is not the only mechanism responsible for the protection by surfactants against hypotonic hemolysis: volume expansion could be compensated via microvesicle release or by the extraction of membrane components upon collisions between red blood cells and surfactant aggregates depending on the membrane composition.
format Articulo
Preprint
author Fait, María Elisa
Hermet, Melisa
Vázquez, Romina Florencia
Maté, Sabina María
Daza Millone, María Antonieta
Vela, María Elena
Morcelle del Valle, Susana Raquel
Bakás, Laura Susana
author_facet Fait, María Elisa
Hermet, Melisa
Vázquez, Romina Florencia
Maté, Sabina María
Daza Millone, María Antonieta
Vela, María Elena
Morcelle del Valle, Susana Raquel
Bakás, Laura Susana
author_sort Fait, María Elisa
title Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
title_short Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
title_full Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
title_fullStr Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
title_full_unstemmed Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
title_sort volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97454
https://ri.conicet.gov.ar/11336/83132
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