Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans

Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as pe...

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Detalles Bibliográficos
Autores principales: Dulbecco, Andrea Belén, Moriconi, Débora Elizabeth, Calderón Fernández, Gustavo Mario, Lynn, María Soledad, McCarthy, Andrés Norman, Roca Acevedo, Gonzalo, Salamanca Moreno, Jhon A., Juárez, Marta Patricia, Pedrini, Nicolás
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97449
https://ri.conicet.gov.ar/11336/83006
https://www.nature.com/articles/s41598-018-28475-x
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97449
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
spellingShingle Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
Dulbecco, Andrea Belén
Moriconi, Débora Elizabeth
Calderón Fernández, Gustavo Mario
Lynn, María Soledad
McCarthy, Andrés Norman
Roca Acevedo, Gonzalo
Salamanca Moreno, Jhon A.
Juárez, Marta Patricia
Pedrini, Nicolás
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
topic_facet Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
description Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.
format Articulo
Articulo
author Dulbecco, Andrea Belén
Moriconi, Débora Elizabeth
Calderón Fernández, Gustavo Mario
Lynn, María Soledad
McCarthy, Andrés Norman
Roca Acevedo, Gonzalo
Salamanca Moreno, Jhon A.
Juárez, Marta Patricia
Pedrini, Nicolás
author_facet Dulbecco, Andrea Belén
Moriconi, Débora Elizabeth
Calderón Fernández, Gustavo Mario
Lynn, María Soledad
McCarthy, Andrés Norman
Roca Acevedo, Gonzalo
Salamanca Moreno, Jhon A.
Juárez, Marta Patricia
Pedrini, Nicolás
author_sort Dulbecco, Andrea Belén
title Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_short Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_full Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_fullStr Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_full_unstemmed Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_sort integument cyp genes of the largest genome-wide cytochrome p450 expansions in triatomines participate in detoxification in deltamethrin-resistant triatoma infestans
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97449
https://ri.conicet.gov.ar/11336/83006
https://www.nature.com/articles/s41598-018-28475-x
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