Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media

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The epidermal growth factor (EGF) plays a key role in physiological and pathological processes. This work reports on the influence of EGF concentration (c EGF) on the modulation of individual cell phenotype and cell colony kinetics with the aim of perturbing the colony front roughness fluctuations....

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Autores principales: Muzzio, Nicolás Eduardo, Carballido, Marcos, Pasquale, Miguel Ángel, González, Pedro Horacio, Azzaroni, Omar, Arvia, Alejandro Jorge
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Física
Biología
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97244
https://ri.conicet.gov.ar/11336/88607
http://stacks.iop.org/1478-3975/15/i=4/a=046001?key=crossref.19bcffde8a4ce797e326c0fd4174018f
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97244
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Física
Biología
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
spellingShingle Física
Biología
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
Muzzio, Nicolás Eduardo
Carballido, Marcos
Pasquale, Miguel Ángel
González, Pedro Horacio
Azzaroni, Omar
Arvia, Alejandro Jorge
Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
topic_facet Física
Biología
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
description The epidermal growth factor (EGF) plays a key role in physiological and pathological processes. This work reports on the influence of EGF concentration (c EGF) on the modulation of individual cell phenotype and cell colony kinetics with the aim of perturbing the colony front roughness fluctuations. For this purpose, HeLa cell colonies that remain confluent along the whole expansion process with initial quasi-radial geometry and different initial cell populations, as well as colonies with initial quasi-linear geometry and large cell population, are employed. Cell size and morphology as well as its adhesive characteristics depend on c EGF. Quasi-radial colonies (QRC) expansion kinetics in EGF-containing medium exhibits a complex behavior. Namely, at the first stages of growth, the average QRC radius evolution can be described by a t 1/2 diffusion term coupled with exponential growth kinetics up to a critical time, and afterwards a growth regime approaching constant velocity. The extension of each regime depends on c EGF and colony history. In the presence of EGF, the initial expansion of quasi-linear colonies (QLCs) also exhibits morphological changes at both the cell and the colony levels. In these cases, the cell density at the colony border region becomes smaller than in the absence of EGF and consequently, the extension of the effective rim where cell duplication and motility contribute to the colony expansion increases. QLC front displacement velocity increases with c EGF up to a maximum value in the 2-10 ng ml-1 range. Individual cell velocity is increased by EGF, and an enhancement in both the persistence and the ballistic characteristics of cell trajectories can be distinguished. For an intermediate c EGF, collective cell displacements contribute to the roughening of the colony contours. This global dynamics becomes compatible with the standard Kardar-Parisi-Zhang growth model, although a faster colony roughness saturation in EGF-containing medium than in the control medium is observed.
format Articulo
Preprint
author Muzzio, Nicolás Eduardo
Carballido, Marcos
Pasquale, Miguel Ángel
González, Pedro Horacio
Azzaroni, Omar
Arvia, Alejandro Jorge
author_facet Muzzio, Nicolás Eduardo
Carballido, Marcos
Pasquale, Miguel Ángel
González, Pedro Horacio
Azzaroni, Omar
Arvia, Alejandro Jorge
author_sort Muzzio, Nicolás Eduardo
title Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_short Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_full Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_fullStr Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_full_unstemmed Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_sort morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/97244
https://ri.conicet.gov.ar/11336/88607
http://stacks.iop.org/1478-3975/15/i=4/a=046001?key=crossref.19bcffde8a4ce797e326c0fd4174018f
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