Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats

Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global isc...

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Autores principales: González Arbeláez, Luisa Fernanda, Ciocci Pardo, Alejandro, Fantinelli, Juliana Catalina, Caldiz, Claudia Irma, Ríos, José Luis, Schinella, Guillermo Raúl, Mosca, Susana María
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Médicas
Infarct size
Mitochondria
Polyphenols
SHR
Wistar
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97156
https://ri.conicet.gov.ar/11336/53924
https://pubs.acs.org/doi/10.1021/acs.jafc.6b01669
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97156
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Infarct size
Mitochondria
Polyphenols
SHR
Wistar
spellingShingle Ciencias Médicas
Infarct size
Mitochondria
Polyphenols
SHR
Wistar
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Fantinelli, Juliana Catalina
Caldiz, Claudia Irma
Ríos, José Luis
Schinella, Guillermo Raúl
Mosca, Susana María
Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats
topic_facet Ciencias Médicas
Infarct size
Mitochondria
Polyphenols
SHR
Wistar
description Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global ischemia, and 60 min reperfusion (R). Other hearts were treated with PCE at the onset of R. Infarct size, the reduced glutathione (GSH), and the expression of phospho-Akt, P-GSK-3β, and P-eNOS were assessed. In isolated mitochondria, the Ca2+-mediated response of mitochondrial permeability transition pore (mPTP), membrane potential (δψm), and superoxide production were determined. PCE decreased infarct size, partly preserved GSH, increased the P-Akt, P-GSK-3β, and P-eNOS contents, improved mPTP response to Ca2+, decreased the superoxide production, and restored δψm. These data show that PCE decreases the cardiac postischemic damage in W rats and SHR and suggest that Akt/GSK-3β/eNOS dependent pathways are involved.
format Articulo
Preprint
author González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Fantinelli, Juliana Catalina
Caldiz, Claudia Irma
Ríos, José Luis
Schinella, Guillermo Raúl
Mosca, Susana María
author_facet González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Fantinelli, Juliana Catalina
Caldiz, Claudia Irma
Ríos, José Luis
Schinella, Guillermo Raúl
Mosca, Susana María
author_sort González Arbeláez, Luisa Fernanda
title Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats
title_short Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats
title_full Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats
title_fullStr Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats
title_full_unstemmed Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats
title_sort ex vivo treatment with a polyphenol-enriched cocoa extract ameliorates myocardial infarct and postischemic mitochondrial injury in normotensive and hypertensive rats
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/97156
https://ri.conicet.gov.ar/11336/53924
https://pubs.acs.org/doi/10.1021/acs.jafc.6b01669
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