A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein

Background: Effector proteins are translocated into host cells by plant-pathogens to undermine pattern-triggeredimmunity (PTI), the plant response to microbe-associated molecular patterns that interferes with the infection process. Individual effectors are found in variable repertoires where some co...

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Autores principales:	Worley, Jay N., Pombo, Marina Alejandra, Zheng, Yi, Dunham, Diane M., Myers, Christopher R., Fei, Zhangjun, Martin, Gregory B.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2016
Materias:	Ciencias Naturales Biología RNA-Seq Pattern-triggered immunity Plant immunity Type III effectors Pseudomonas syringae
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/97056 https://ri.conicet.gov.ar/11336/84373 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2534-4
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-97056
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Biología RNA-Seq Pattern-triggered immunity Plant immunity Type III effectors Pseudomonas syringae
spellingShingle	Ciencias Naturales Biología RNA-Seq Pattern-triggered immunity Plant immunity Type III effectors Pseudomonas syringae Worley, Jay N. Pombo, Marina Alejandra Zheng, Yi Dunham, Diane M. Myers, Christopher R. Fei, Zhangjun Martin, Gregory B. A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
topic_facet	Ciencias Naturales Biología RNA-Seq Pattern-triggered immunity Plant immunity Type III effectors Pseudomonas syringae
description	Background: Effector proteins are translocated into host cells by plant-pathogens to undermine pattern-triggeredimmunity (PTI), the plant response to microbe-associated molecular patterns that interferes with the infection process. Individual effectors are found in variable repertoires where some constituents target the same pathways.The effector protein AvrPto from <i>Pseudomonas syringae</i> has a core domain (CD) and C-terminal domain (CTD) that each promotes bacterial growth and virulence in tomato. The individual contributions of each domain and whether they act redundantly is unknown. Results: We use RNA-Seq to elucidate the contribution of the CD and CTD to the suppression of PTI in tomato leaves 6 h after inoculation. Unexpectedly, each domain alters transcript levels of essentially the same genes but to a different degree. This difference, when quantified, reveals that although targeting the same host genes, the two domains act synergistically. AvrPto has a relatively greater effect on genes whose expression is suppressed during PTI, and the effect on these genes appears to be diminished by saturation. Conclusions: RNA-Seq profiles can be used to observe relative contributions of effector subdomains to PTI suppression. Our analysis shows the CD and CTD multiplicatively affect the same gene transcript levels with a greater relative impact on genes whose expression is suppressed during PTI. The higher degree of up-regulation versus down-regulation during PTI is plausibly an evolutionary adaptation against effectors that target immune signaling
format	Articulo Articulo
author	Worley, Jay N. Pombo, Marina Alejandra Zheng, Yi Dunham, Diane M. Myers, Christopher R. Fei, Zhangjun Martin, Gregory B.
author_facet	Worley, Jay N. Pombo, Marina Alejandra Zheng, Yi Dunham, Diane M. Myers, Christopher R. Fei, Zhangjun Martin, Gregory B.
author_sort	Worley, Jay N.
title	A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
title_short	A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
title_full	A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
title_fullStr	A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
title_full_unstemmed	A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
title_sort	novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/97056 https://ri.conicet.gov.ar/11336/84373 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2534-4
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A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein

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