Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B

Aim: To analyze the physiological response of Sphingobium sp. 22B to water stress. Methods and results:The strain was grown under excess of carbon source and then submitted to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (P...

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Autores principales: Madueño, Laura, Coppotelli, Bibiana Marina, Festa, Sabrina, Alvarez, H. M., Morelli, Irma Susana
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Química
PAH-degrading sphingomonas
Sphingobium sp. 22B
Desiccation
Water stress
Patagonia
PHB
Glycogen
Tehalose
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/96974
https://ri.conicet.gov.ar/11336/90677
http://doi.wiley.com/10.1111/jam.13742
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-96974
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
PAH-degrading sphingomonas
Sphingobium sp. 22B
Desiccation
Water stress
Patagonia
PHB
Glycogen
Tehalose
spellingShingle Química
PAH-degrading sphingomonas
Sphingobium sp. 22B
Desiccation
Water stress
Patagonia
PHB
Glycogen
Tehalose
Madueño, Laura
Coppotelli, Bibiana Marina
Festa, Sabrina
Alvarez, H. M.
Morelli, Irma Susana
Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B
topic_facet Química
PAH-degrading sphingomonas
Sphingobium sp. 22B
Desiccation
Water stress
Patagonia
PHB
Glycogen
Tehalose
description Aim: To analyze the physiological response of Sphingobium sp. 22B to water stress. Methods and results:The strain was grown under excess of carbon source and then submitted to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (PHB), and transmission electron microscopy (TEM) was studied. Genes linked with desiccation were searched in Sphingobium sp. 22B and Sphingomonas "sensu latu" genomes and their transcripts were quantified by Real-Time PCR. Results showed that, in absence of water stress, strain 22B accumulated 4.76± 1.41% of glycogen, 0.84± 1.62% of trehalose and 44.9± 6.4% of PHB per cellular dry weight. Glycogen and trehalose were mobilized in water stresses conditions, this mobilization was significantly higher in 60RH in comparison to 18RH. Gene treY was upregulated 6-fold change in 60RH relative to 18RH. TEM and quantification of PHB revealed that PHB was mobilized under 60RH condition accompanied by the downregulation of the phbB gene. TEM images showed an extracellular amorphous matrix in 18RH and 60RH. Major differences were found in the presence of aqpZ and trehalose genes between strain 22B and Sphingomonas genomes. Conclusion: Strain 22B showed a carbon conservative metabolism capable of accumulation of three types of endogenous carbon sources. The strain responds to water stress by changing the expression pattern of genes related with desiccation, formation of an extracellular amorphous matrix and mobilization of the carbon sources according to the degree of water stress. Trehalose, glycogen and PHB may have multiple functions in different degrees of desiccation. The robust endowment of molecular responses to desiccation shown in Sphingobium sp. 22B could explain its survival in semiarid soil.Significance and Impact of the studyUnderstanding the physiology implicated in the toleration of the PAH-degrading strain Sphingobium sp 22B to environmental desiccation may improve the bioaugmentation technologies in semiarid hydrocarbons contaminated soils.
format Articulo
Preprint
author Madueño, Laura
Coppotelli, Bibiana Marina
Festa, Sabrina
Alvarez, H. M.
Morelli, Irma Susana
author_facet Madueño, Laura
Coppotelli, Bibiana Marina
Festa, Sabrina
Alvarez, H. M.
Morelli, Irma Susana
author_sort Madueño, Laura
title Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B
title_short Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B
title_full Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B
title_fullStr Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B
title_full_unstemmed Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B
title_sort insights into the mechanisms of desiccation resistance of the patagonian pah-degrading strain sphingobium sp. 22b
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/96974
https://ri.conicet.gov.ar/11336/90677
http://doi.wiley.com/10.1111/jam.13742
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