Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles

Riboflavin (Rf) is an endogenous photosensitizer, which can participate in Type I and Type II processes. We have recently shown that the yield of the triplet excited states of Rf is enhanced in the presence of pectin-coated silver nanoparticles (Pec@AgNP) due to formation of a complex between Rf and...

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Detalles Bibliográficos
Autores principales:	Rivas Aiello, María Belén, Castrogiovanni, Daniel Cayetano, Parisi, Julieta Marcia, Azcárate, Julio César, García Einschlag, Fernando Sebastián, Gensch, Thomas, Bosio, Gabriela Natalia, Mártire, Daniel Osvaldo
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2018
Materias:	Ciencias Exactas Rojo neutro Mtt Riboflavine
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/96850 https://ri.conicet.gov.ar/11336/88730 https://onlinelibrary.wiley.com/doi/full/10.1111/php.12974
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-96850
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Rojo neutro Mtt Riboflavine
spellingShingle	Ciencias Exactas Rojo neutro Mtt Riboflavine Rivas Aiello, María Belén Castrogiovanni, Daniel Cayetano Parisi, Julieta Marcia Azcárate, Julio César García Einschlag, Fernando Sebastián Gensch, Thomas Bosio, Gabriela Natalia Mártire, Daniel Osvaldo Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
topic_facet	Ciencias Exactas Rojo neutro Mtt Riboflavine
description	Riboflavin (Rf) is an endogenous photosensitizer, which can participate in Type I and Type II processes. We have recently shown that the yield of the triplet excited states of Rf is enhanced in the presence of pectin-coated silver nanoparticles (Pec@AgNP) due to formation of a complex between Rf and Pec@AgNP (Rf-Pec@AgNP). Consequently, under aerobic conditions, the amounts of singlet molecular oxygen and superoxide radical anion generated are also larger in the presence of the nanoparticles. This result made us suspect that the nanoparticles could have a beneficial effect in Rf-based PDT. To prove this hypothesis, we here compared the photodamage in HeLa cells incubated with Rf in the presence and in the absence of Pec@AgNP applying several optical assays. We used fluorescence imaging of irradiated HeLa cells incubated with Annexin V and propidium iodide to evaluate the occurrence of apoptosis/necrosis, the reduction of the tetrazolium dye MTT to formazan and neutral red uptake to prove cell viability, as well as synchrotron infrared microscopy of single cells to evaluate possible structural changes of DNA and nuclear proteins. The enhanced photodamage observed in the presence of Pec@AgNP seems to indicate that Rf enters into the cells complexed with the nanoparticles.
format	Articulo Preprint
author	Rivas Aiello, María Belén Castrogiovanni, Daniel Cayetano Parisi, Julieta Marcia Azcárate, Julio César García Einschlag, Fernando Sebastián Gensch, Thomas Bosio, Gabriela Natalia Mártire, Daniel Osvaldo
author_facet	Rivas Aiello, María Belén Castrogiovanni, Daniel Cayetano Parisi, Julieta Marcia Azcárate, Julio César García Einschlag, Fernando Sebastián Gensch, Thomas Bosio, Gabriela Natalia Mártire, Daniel Osvaldo
author_sort	Rivas Aiello, María Belén
title	Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
title_short	Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
title_full	Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
title_fullStr	Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
title_full_unstemmed	Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles
title_sort	photodynamic therapy in hela cells incubated with riboflavin and pectin-coated silver nanoparticles
publishDate	2018
url	http://sedici.unlp.edu.ar/handle/10915/96850 https://ri.conicet.gov.ar/11336/88730 https://onlinelibrary.wiley.com/doi/full/10.1111/php.12974
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Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin-coated Silver Nanoparticles

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