Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties

Polymerized liposomes encapsulating L-tryptophan were studied with the aim to characterize them as drug delivery systems for the treatment of several metabolic diseases that need an increased systemic L-tryptophan concentration, polymerized liposomes were obtained by UV irradiation of vesicles conta...

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Autores principales: Fernández Ruocco, María Julieta, Siri, Macarena, Igartúa, Daniela Edith, Prieto, María Jimena, Alonso, Silvia del Valle, Chiaramoni, Nadia Silvia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Médicas
Ingeniería
Lipopolymers
L-tryptophan
Drug delivery
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/96392
https://ri.conicet.gov.ar/11336/77442
https://www.scirp.org/journal/PaperInformation.aspx?PaperID=29497
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-96392
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Ingeniería
Lipopolymers
L-tryptophan
Drug delivery
spellingShingle Ciencias Médicas
Ingeniería
Lipopolymers
L-tryptophan
Drug delivery
Fernández Ruocco, María Julieta
Siri, Macarena
Igartúa, Daniela Edith
Prieto, María Jimena
Alonso, Silvia del Valle
Chiaramoni, Nadia Silvia
Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties
topic_facet Ciencias Médicas
Ingeniería
Lipopolymers
L-tryptophan
Drug delivery
description Polymerized liposomes encapsulating L-tryptophan were studied with the aim to characterize them as drug delivery systems for the treatment of several metabolic diseases that need an increased systemic L-tryptophan concentration, polymerized liposomes were obtained by UV irradiation of vesicles containing 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in a 1:1 molar ratio, in the presence of 10 and 50 mol% of L-tryptophan (respect to total lipid concentration). Polymerization efficiency was studied spectrophotometrically. Also, bilayer packing at the polar head region was followed with the Merocyanine 540 (MC540) and specific interactions in the lipopolymers were studied by FTIR. High L-tryptophan concentrations (50 mol% respect to total lipid concentration) induced a higher amount of six- and nine-unit polymers. This phenomenon was induced because the L-tryptophan located outside the lipid membrane was included in it during the polymerization process and was thus responsible for the better accommodate of the polar head region. This was not possible with the lower amount of L-tryptophan (10 mol%). The stability of lipopolymers with different amounts of L-tryptophan was studied through release profiles. Polymerized liposomes with 50 mol% of L-tryptophan were able to retain around 80% of the amino acid after 24 hours, whereas those with 10 mol % of the amino acid were able to retain 20%. The metabolic activity of the Caco-2 cell line was also studied. Cytotoxic effects were low in the presence of polymerized liposomes, rendering a maximum percentage of cell death of 30%. In summary, this work stresses the relevance of nonspecific drug-polymerized membrane binding on L-tryptophan pharmacological interaction with possible pharmaceutical applications in liposomal drug delivery. Moreover, the absence of significant cytotoxic effects allows the system proposed to be applied in human health.
format Articulo
Articulo
author Fernández Ruocco, María Julieta
Siri, Macarena
Igartúa, Daniela Edith
Prieto, María Jimena
Alonso, Silvia del Valle
Chiaramoni, Nadia Silvia
author_facet Fernández Ruocco, María Julieta
Siri, Macarena
Igartúa, Daniela Edith
Prieto, María Jimena
Alonso, Silvia del Valle
Chiaramoni, Nadia Silvia
author_sort Fernández Ruocco, María Julieta
title Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties
title_short Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties
title_full Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties
title_fullStr Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties
title_full_unstemmed Lipid-polymer membranes as carriers for L-tryptophan : Molecular and metabolic properties
title_sort lipid-polymer membranes as carriers for l-tryptophan : molecular and metabolic properties
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/96392
https://ri.conicet.gov.ar/11336/77442
https://www.scirp.org/journal/PaperInformation.aspx?PaperID=29497
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