Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-loca...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2013
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/96269 https://ri.conicet.gov.ar/11336/75838 https://www.tandfonline.com/doi/abs/10.4161/trns.27672 |
| Aporte de: |
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I19-R120-10915-96269 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Ciencias Naturales Co-transcriptional splicing Hox genes Nuclear actin Nuclear speckles Rna pol ii Transcription/splicing |
| spellingShingle |
Biología Ciencias Naturales Co-transcriptional splicing Hox genes Nuclear actin Nuclear speckles Rna pol ii Transcription/splicing Naum-Onganía, Gabriela Díaz Cortés, Víctor Manuel Blasi, Francesco Rivera Pomar, Rolando Víctor Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved? |
| topic_facet |
Biología Ciencias Naturales Co-transcriptional splicing Hox genes Nuclear actin Nuclear speckles Rna pol ii Transcription/splicing |
| description |
The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WAS P, and transcription/splicing factors p54<sup>nrb</sup> and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles. |
| format |
Articulo Articulo |
| author |
Naum-Onganía, Gabriela Díaz Cortés, Víctor Manuel Blasi, Francesco Rivera Pomar, Rolando Víctor |
| author_facet |
Naum-Onganía, Gabriela Díaz Cortés, Víctor Manuel Blasi, Francesco Rivera Pomar, Rolando Víctor |
| author_sort |
Naum-Onganía, Gabriela |
| title |
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved? |
| title_short |
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved? |
| title_full |
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved? |
| title_fullStr |
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved? |
| title_full_unstemmed |
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved? |
| title_sort |
nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : are nuclear speckles involved? |
| publishDate |
2013 |
| url |
http://sedici.unlp.edu.ar/handle/10915/96269 https://ri.conicet.gov.ar/11336/75838 https://www.tandfonline.com/doi/abs/10.4161/trns.27672 |
| work_keys_str_mv |
AT naumonganiagabriela nuclearactinpolymerizationfromfastergrowingendsintheinitialactivationofhoxgenetranscriptionarenuclearspecklesinvolved AT diazcortesvictormanuel nuclearactinpolymerizationfromfastergrowingendsintheinitialactivationofhoxgenetranscriptionarenuclearspecklesinvolved AT blasifrancesco nuclearactinpolymerizationfromfastergrowingendsintheinitialactivationofhoxgenetranscriptionarenuclearspecklesinvolved AT riverapomarrolandovictor nuclearactinpolymerizationfromfastergrowingendsintheinitialactivationofhoxgenetranscriptionarenuclearspecklesinvolved |
| bdutipo_str |
Repositorios |
| _version_ |
1764820491955077123 |