Digital processing of in situ hybridization images: identification and spatial allocation of specific labels
In situ hybridization (ISH) method allows to reveal specific genes expression, identify specific cell types and detect areas or tissues, displaying differential gene expression. This work describes a standardized procedure of digital image processing that allows detailed analyses of ISH preparations...
Guardado en:
Autores principales: | , , , , |
---|---|
Formato: | Articulo |
Lenguaje: | Inglés |
Publicado: |
2007
|
Materias: | |
Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/9563 http://journal.info.unlp.edu.ar/wp-content/uploads/JCST-Oct07-9.pdf |
Aporte de: |
id |
I19-R120-10915-9563 |
---|---|
record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Informáticas Segmentation digital image processing |
spellingShingle |
Ciencias Informáticas Segmentation digital image processing González, Mariela A. Rapacioli, Melina Ballarín, Virginia Laura Fiszer de Plazas, Sara Flores, Vladimir Digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
topic_facet |
Ciencias Informáticas Segmentation digital image processing |
description |
In situ hybridization (ISH) method allows to reveal specific genes expression, identify specific cell types and detect areas or tissues, displaying differential gene expression. This work describes a standardized procedure of digital image processing that allows detailed analyses of ISH preparations. We have developed a software that allows through a graphical interface (a) to reliably identify and quantify ISH labels, (b) to locate each label within the image reference system (c) to assemble the total series of images obtained from a complete histological sections of a biological structure, and d) to locate all the labels within a unique reference system that corresponds to the complete biological structure. As each ISH label is positioned within a spatial coordinates system coinciding with an intrinsic biological reference axis, this software can be used to analyze the spatial pattern of distribution of specific genes expression during the embryonic development. The software allows the construction of numerical space series that can be used to analyze the variability and the dynamics of genes expression as a function of space and/or time during the embryonic development. |
format |
Articulo Articulo |
author |
González, Mariela A. Rapacioli, Melina Ballarín, Virginia Laura Fiszer de Plazas, Sara Flores, Vladimir |
author_facet |
González, Mariela A. Rapacioli, Melina Ballarín, Virginia Laura Fiszer de Plazas, Sara Flores, Vladimir |
author_sort |
González, Mariela A. |
title |
Digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
title_short |
Digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
title_full |
Digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
title_fullStr |
Digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
title_full_unstemmed |
Digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
title_sort |
digital processing of in situ hybridization images: identification and spatial allocation of specific labels |
publishDate |
2007 |
url |
http://sedici.unlp.edu.ar/handle/10915/9563 http://journal.info.unlp.edu.ar/wp-content/uploads/JCST-Oct07-9.pdf |
work_keys_str_mv |
AT gonzalezmarielaa digitalprocessingofinsituhybridizationimagesidentificationandspatialallocationofspecificlabels AT rapaciolimelina digitalprocessingofinsituhybridizationimagesidentificationandspatialallocationofspecificlabels AT ballarinvirginialaura digitalprocessingofinsituhybridizationimagesidentificationandspatialallocationofspecificlabels AT fiszerdeplazassara digitalprocessingofinsituhybridizationimagesidentificationandspatialallocationofspecificlabels AT floresvladimir digitalprocessingofinsituhybridizationimagesidentificationandspatialallocationofspecificlabels |
bdutipo_str |
Repositorios |
_version_ |
1764820492177375233 |