Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)

In an attempt to track the chromosomal differentiation in the Dichroplus elongatus species group, we analyzed the karyotypes of four species with classical cytogenetic and mapping several multigene families through fluorescent in situ hybridization (FISH). We improved the taxon sampling of the D. el...

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Autores principales: Castillo, Elio Rodrigo Daniel, Taffarel, Alberto, Maronna, Maximiliano M., Cigliano, María Marta, Palacios Gimenez, Octavio Manuel, Cabral de Mello, Diogo C., Marti, Dardo Andrea
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/93303
https://ri.conicet.gov.ar/handle/11336/63133
Aporte de:
id I19-R120-10915-93303
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Dichroplus elongatus species group
Chromosome evolution
Phylogeny
spellingShingle Ciencias Naturales
Dichroplus elongatus species group
Chromosome evolution
Phylogeny
Castillo, Elio Rodrigo Daniel
Taffarel, Alberto
Maronna, Maximiliano M.
Cigliano, María Marta
Palacios Gimenez, Octavio Manuel
Cabral de Mello, Diogo C.
Marti, Dardo Andrea
Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)
topic_facet Ciencias Naturales
Dichroplus elongatus species group
Chromosome evolution
Phylogeny
description In an attempt to track the chromosomal differentiation in the Dichroplus elongatus species group, we analyzed the karyotypes of four species with classical cytogenetic and mapping several multigene families through fluorescent in situ hybridization (FISH). We improved the taxon sampling of the D. elongatus species group adding new molecular data to infer the phylogeny of the genus and reconstruct the karyotype evolution. Our molecular analyses recovered a fully resolved tree with no evidence for the monophyly of Dichroplus. However, we recovered several stable clades within the genus, including the D. elongatus species group, under the different strategies of tree analyses (Maximum Parsimony and Maximum Likelihood). The chromosomal data revealed minor variation in the D. elongatus species group's karyotypes caused by chromosome rearrangements compared to the phylogenetically related D. maculipennis species group. The karyotypes of D. intermedius and D. exilis described herein showed the standard characteristics found in most Dichroplini, 2n = 23/24, X0♂ XX♀, Fundamental number (FN) = 23/24. However, we noticed two established pericentric inversions in D. intermedius karyotype, raising the FN to 27♂/28♀. A strong variation in the heterochromatic blocks distribution was evidenced at interespecific level. The multigene families' mapping revealed significant variation, mainly in rDNA clusters. These variations are probably caused by micro chromosomal changes, such as movement of transposable elements (TEs) and ectopic recombination. These observations suggest a high genomic dynamism for these repetitive DNA sequences in related species. The reconstruction of the chromosome character "variation in the FN" posits the FN = 23/24 as the ancestral state, and it is hypothesized that variations due to pericentric inversions has arisen independently three times in the evolutionary history of Dichroplus. One of these independent events occurred in the D. elongatus species group, where D. intermedius is the unique case with the highest FN described in the tribe Dichroplini.
format Articulo
Articulo
author Castillo, Elio Rodrigo Daniel
Taffarel, Alberto
Maronna, Maximiliano M.
Cigliano, María Marta
Palacios Gimenez, Octavio Manuel
Cabral de Mello, Diogo C.
Marti, Dardo Andrea
author_facet Castillo, Elio Rodrigo Daniel
Taffarel, Alberto
Maronna, Maximiliano M.
Cigliano, María Marta
Palacios Gimenez, Octavio Manuel
Cabral de Mello, Diogo C.
Marti, Dardo Andrea
author_sort Castillo, Elio Rodrigo Daniel
title Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)
title_short Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)
title_full Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)
title_fullStr Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)
title_full_unstemmed Phylogeny and chromosomal diversification in the <i>Dichroplus elongatus</i> species group (Orthoptera, Melanoplinae)
title_sort phylogeny and chromosomal diversification in the <i>dichroplus elongatus</i> species group (orthoptera, melanoplinae)
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/93303
https://ri.conicet.gov.ar/handle/11336/63133
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