First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurolo...

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Autores principales: Serena, María Soledad, Metz, Germán Ernesto, Lozada, María Inés, Aspitia, Carolina Gabriela, Nicolino, Edgardo Héctor, Pidone, Claudio Luis, Fossaroli, Melisa Gisele, Balsalobre, Agustín, Quiroga, María Alejandra, Echeverría, María Gabriela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/93228
https://ri.conicet.gov.ar/handle/11336/94583
https://www.ajol.info/index.php/ovj/article/view/169608
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-93228
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
spellingShingle Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
Serena, María Soledad
Metz, Germán Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Héctor
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustín
Quiroga, María Alejandra
Echeverría, María Gabriela
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
topic_facet Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
description Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
format Articulo
Articulo
author Serena, María Soledad
Metz, Germán Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Héctor
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustín
Quiroga, María Alejandra
Echeverría, María Gabriela
author_facet Serena, María Soledad
Metz, Germán Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Héctor
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustín
Quiroga, María Alejandra
Echeverría, María Gabriela
author_sort Serena, María Soledad
title First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_short First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_full First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_fullStr First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_full_unstemmed First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_sort first isolation and molecular characterization of suid herpesvirus type 1 from a domestic dog in argentina
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/93228
https://ri.conicet.gov.ar/handle/11336/94583
https://www.ajol.info/index.php/ovj/article/view/169608
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