Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts

Papain from latex of Carica papaya was purified up to matrix-assisted laser desorption= ionization (MALDI) time-of-flight (TOF) mass spectrometry homogeneity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium...

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Autores principales: Llerena Suster, Carlos Rafael, Priolo de Lufrano, Nora Silvia, Morcelle del Valle, Susana Raquel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/92383
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-92383
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
spellingShingle Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
Llerena Suster, Carlos Rafael
Priolo de Lufrano, Nora Silvia
Morcelle del Valle, Susana Raquel
Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
topic_facet Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
description Papain from latex of Carica papaya was purified up to matrix-assisted laser desorption= ionization (MALDI) time-of-flight (TOF) mass spectrometry homogeneity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4%) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain on a preparative scale.
format Articulo
Articulo
author Llerena Suster, Carlos Rafael
Priolo de Lufrano, Nora Silvia
Morcelle del Valle, Susana Raquel
author_facet Llerena Suster, Carlos Rafael
Priolo de Lufrano, Nora Silvia
Morcelle del Valle, Susana Raquel
author_sort Llerena Suster, Carlos Rafael
title Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_short Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_full Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_fullStr Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_full_unstemmed Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_sort sodium tetrathionate effect on papain purification from different <i>carica papaya</i> latex crude extracts
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/92383
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