Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>

Latex fractions from Calotropis procera, Cryptostegia grandiflora, Plumeria rubra, and Himatanthus drasticus were assayed in order to prospect for new plant peptidases with milk-clotting activities, for use as rennet alternatives. Only C. procera and C. grandiflora latex fractions exhibited proteoly...

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Autores principales:	Freitas, Cleverson D. T., Leite, Hugo B., Oliveira, João B. P., Amaral, Jackson L., Egito, Antônio S., Vairo Cavalli, Sandra Elizabeth, Lobo, Marina D. P., Monteiro Moreira, Ana C. O., Ramos, Márcio V.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2016
Materias:	Biología Casein Chymosin Himatanthus drasticus Plumeria rubra Papain-like peptidase Rennet
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/92378
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-92378
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Casein Chymosin Himatanthus drasticus Plumeria rubra Papain-like peptidase Rennet
spellingShingle	Biología Casein Chymosin Himatanthus drasticus Plumeria rubra Papain-like peptidase Rennet Freitas, Cleverson D. T. Leite, Hugo B. Oliveira, João B. P. Amaral, Jackson L. Egito, Antônio S. Vairo Cavalli, Sandra Elizabeth Lobo, Marina D. P. Monteiro Moreira, Ana C. O. Ramos, Márcio V. Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>
topic_facet	Biología Casein Chymosin Himatanthus drasticus Plumeria rubra Papain-like peptidase Rennet
description	Latex fractions from Calotropis procera, Cryptostegia grandiflora, Plumeria rubra, and Himatanthus drasticus were assayed in order to prospect for new plant peptidases with milk-clotting activities, for use as rennet alternatives. Only C. procera and C. grandiflora latex fractions exhibited proteolytic and milk-clotting activities, which were not affected by high concentrations of NaCl and CaCl2. However, pre-incubation of both samples at 75 °C for 10 min eliminated completely their activities. Both proteolytic fractions were able to hydrolyze k-casein and to produce peptides of 16 kDa, a similar SDS-PAGE profile to commercial chymosin. RP-HPLC and mass spectrometry analyses of the k-casein peptides showed that the peptidases from C. procera or C. grandiflora hydrolyzed k-casein similar to commercial chymosin. The cheeses made with both latex peptidases exhibited yields, dry masses, and soluble proteins similar to cheeses prepared with commercial chymosin. In conclusion, C. procera and C. grandiflora latex peptidases with the ability to coagulate milk can be used as alternatives to commercial animal chymosin in the cheese manufacturing process.
format	Articulo Articulo
author	Freitas, Cleverson D. T. Leite, Hugo B. Oliveira, João B. P. Amaral, Jackson L. Egito, Antônio S. Vairo Cavalli, Sandra Elizabeth Lobo, Marina D. P. Monteiro Moreira, Ana C. O. Ramos, Márcio V.
author_facet	Freitas, Cleverson D. T. Leite, Hugo B. Oliveira, João B. P. Amaral, Jackson L. Egito, Antônio S. Vairo Cavalli, Sandra Elizabeth Lobo, Marina D. P. Monteiro Moreira, Ana C. O. Ramos, Márcio V.
author_sort	Freitas, Cleverson D. T.
title	Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>
title_short	Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>
title_full	Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>
title_fullStr	Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>
title_full_unstemmed	Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>
title_sort	insights into milk-clotting activity of latex peptidases from <i>calotropis procera</i> and <i>cryptostegia grandiflora</i>
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/92378
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Insights into milk-clotting activity of latex peptidases from <i>Calotropis procera</i> and <i>Cryptostegia grandiflora</i>

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