Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses

Papain was purified from dried Carica papaya latex by fractioned salt precipitation in presence of sodium tetrathionate to preserve enzymatic activity. Purification was followed by different electrophoretic methods. Identification of the purified product was afforded by submitting the peptides obtai...

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Detalles Bibliográficos
Autores principales: Llerena Suster, Carlos Rafael, Obregón, Walter David, Trejo, Sebastián Alejandro, Morcelle del Valle, Susana Raquel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Biología
Caricain
Chymopapain
Glycyl endopeptidase
MALDI-TOF
Peptide mass fingerprint
Papain
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/92371
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-92371
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Caricain
Chymopapain
Glycyl endopeptidase
MALDI-TOF
Peptide mass fingerprint
Papain
spellingShingle Biología
Caricain
Chymopapain
Glycyl endopeptidase
MALDI-TOF
Peptide mass fingerprint
Papain
Llerena Suster, Carlos Rafael
Obregón, Walter David
Trejo, Sebastián Alejandro
Morcelle del Valle, Susana Raquel
Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses
topic_facet Biología
Caricain
Chymopapain
Glycyl endopeptidase
MALDI-TOF
Peptide mass fingerprint
Papain
description Papain was purified from dried Carica papaya latex by fractioned salt precipitation in presence of sodium tetrathionate to preserve enzymatic activity. Purification was followed by different electrophoretic methods. Identification of the purified product was afforded by submitting the peptides obtained by tryptic digestion of papain to matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS) analysis. Comparison of the peptide masses analyzed by peptide mass fingerprinting (PMF) MALDI-TOF and those obtained by theoretical tryptic digestion, revealed the presence of some peptides belonging the other three endopeptidases contained in papaya latex (very similar to papain in molecular weight and pI) in the purified fraction of papain. The PMF by MALDI-TOF could be applied as a method to follow papain purification.
format Articulo
Articulo
author Llerena Suster, Carlos Rafael
Obregón, Walter David
Trejo, Sebastián Alejandro
Morcelle del Valle, Susana Raquel
author_facet Llerena Suster, Carlos Rafael
Obregón, Walter David
Trejo, Sebastián Alejandro
Morcelle del Valle, Susana Raquel
author_sort Llerena Suster, Carlos Rafael
title Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses
title_short Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses
title_full Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses
title_fullStr Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses
title_full_unstemmed Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses
title_sort papain purification insights: monitoring by electrophoretic approaches and maldi-tof peptide mass fingerprint analyses
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/92371
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