Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtai...
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| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1964
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/89935 |
| Aporte de: |
| Sumario: | Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that physalaemin is an endecapeptide having the following amino acid composition and sequence: Pyr-Ala-Asp(OH)-Pro-Asp(NH2)-Lys-Phe-Tyr-Gly-Leu-Met-NH2 Some anomalies in the trypsin and chymotrypsin fission of physalaemin are discussed, and the strict chemical resemblance between physalaemin and eledoisin is pointed out. |
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