Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus

Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtai...

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Detalles Bibliográficos
Autores principales: Anastasi, A., Erspamer, V., Cei, José Miguel Alfredo María
Formato: Articulo
Lenguaje:Inglés
Publicado: 1964
Materias:
Zoología
Anfibios
Polypeptide physalaemin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/89935
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-89935
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Zoología
Anfibios
Polypeptide physalaemin
spellingShingle Zoología
Anfibios
Polypeptide physalaemin
Anastasi, A.
Erspamer, V.
Cei, José Miguel Alfredo María
Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
topic_facet Zoología
Anfibios
Polypeptide physalaemin
description Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that physalaemin is an endecapeptide having the following amino acid composition and sequence: Pyr-Ala-Asp(OH)-Pro-Asp(NH2)-Lys-Phe-Tyr-Gly-Leu-Met-NH2 Some anomalies in the trypsin and chymotrypsin fission of physalaemin are discussed, and the strict chemical resemblance between physalaemin and eledoisin is pointed out.
format Articulo
Articulo
author Anastasi, A.
Erspamer, V.
Cei, José Miguel Alfredo María
author_facet Anastasi, A.
Erspamer, V.
Cei, José Miguel Alfredo María
author_sort Anastasi, A.
title Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_short Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_full Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_fullStr Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_full_unstemmed Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_sort isolation and amino acid sequence of physalaemin, the main active polypeptide of the skin of physalaemus fuscumaculatus
publishDate 1964
url http://sedici.unlp.edu.ar/handle/10915/89935
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