Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms pre...

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Autores principales: Festa, Sabrina, Coppotelli, Bibiana Marina, Madueño, Laura, Loviso, Claudia Lorena, Macchi, Marianela, Neme Tauil, Ricardo Martín, Valacco, María Pía, Morelli, Irma Susana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Naturales
Bacterias
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87764
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87764
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Bacterias
spellingShingle Ciencias Naturales
Bacterias
Festa, Sabrina
Coppotelli, Bibiana Marina
Madueño, Laura
Loviso, Claudia Lorena
Macchi, Marianela
Neme Tauil, Ricardo Martín
Valacco, María Pía
Morelli, Irma Susana
Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
topic_facet Ciencias Naturales
Bacterias
description The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31–43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).
format Articulo
Articulo
author Festa, Sabrina
Coppotelli, Bibiana Marina
Madueño, Laura
Loviso, Claudia Lorena
Macchi, Marianela
Neme Tauil, Ricardo Martín
Valacco, María Pía
Morelli, Irma Susana
author_facet Festa, Sabrina
Coppotelli, Bibiana Marina
Madueño, Laura
Loviso, Claudia Lorena
Macchi, Marianela
Neme Tauil, Ricardo Martín
Valacco, María Pía
Morelli, Irma Susana
author_sort Festa, Sabrina
title Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
title_short Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
title_full Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
title_fullStr Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
title_full_unstemmed Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
title_sort assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87764
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