Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis

Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the <i>de novo</i> glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported...

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Detalles Bibliográficos
Autores principales: García Fabiani, María Belén, Montanaro, Mauro Aldo, Stringa, Pablo, Lacunza, Ezequiel, Cattaneo, Elizabeth Renee, Santana, Marianela, Pellón Maisón, Magalí, González Baró, María del Rosario
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Médicas
Meiosis
Apoptosis
mouse
gene silencing
germ cell
GPAT2
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87730
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87730
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Meiosis
Apoptosis
mouse
gene silencing
germ cell
GPAT2
spellingShingle Ciencias Médicas
Meiosis
Apoptosis
mouse
gene silencing
germ cell
GPAT2
García Fabiani, María Belén
Montanaro, Mauro Aldo
Stringa, Pablo
Lacunza, Ezequiel
Cattaneo, Elizabeth Renee
Santana, Marianela
Pellón Maisón, Magalí
González Baró, María del Rosario
Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
topic_facet Ciencias Médicas
Meiosis
Apoptosis
mouse
gene silencing
germ cell
GPAT2
description Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the <i>de novo</i> glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear. The aim of the present study was to confirm the hypothesis that GPAT2 is required for the normal physiology of testes and male germ cell maturation. The gene was silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hairpin RNA targeting <i>Gpat2</i> mRNA into mouse testis. Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness were also observed, and the analysis of apoptosis-related gene expression demonstrated the activation of apoptosis in <i>Gpat2</i>-silenced germ cells. These findings indicate that GPAT2 protein is necessary for the normal development of male gonocytes, and that its absence triggers apoptotic mechanisms, thereby decreasing the number of dividing germ cells.
format Articulo
Preprint
author García Fabiani, María Belén
Montanaro, Mauro Aldo
Stringa, Pablo
Lacunza, Ezequiel
Cattaneo, Elizabeth Renee
Santana, Marianela
Pellón Maisón, Magalí
González Baró, María del Rosario
author_facet García Fabiani, María Belén
Montanaro, Mauro Aldo
Stringa, Pablo
Lacunza, Ezequiel
Cattaneo, Elizabeth Renee
Santana, Marianela
Pellón Maisón, Magalí
González Baró, María del Rosario
author_sort García Fabiani, María Belén
title Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
title_short Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
title_full Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
title_fullStr Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
title_full_unstemmed Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
title_sort glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87730
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