The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features

The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Latorre Estivalis, José Manuel, Robertson, H. M., Walden, K. K. O., Ruiz, J., Gonçalves, L.O., Guarneri, A. A., Lorenzo, M. G.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87711
Aporte de:
id I19-R120-10915-87711
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
spellingShingle Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
Latorre Estivalis, José Manuel
Robertson, H. M.
Walden, K. K. O.
Ruiz, J.
Gonçalves, L.O.
Guarneri, A. A.
Lorenzo, M. G.
The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
topic_facet Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
description The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5<sup>th</sup> instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
format Articulo
Articulo
author Latorre Estivalis, José Manuel
Robertson, H. M.
Walden, K. K. O.
Ruiz, J.
Gonçalves, L.O.
Guarneri, A. A.
Lorenzo, M. G.
author_facet Latorre Estivalis, José Manuel
Robertson, H. M.
Walden, K. K. O.
Ruiz, J.
Gonçalves, L.O.
Guarneri, A. A.
Lorenzo, M. G.
author_sort Latorre Estivalis, José Manuel
title The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_short The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_full The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_fullStr The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_full_unstemmed The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_sort molecular sensory machinery of a chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87711
work_keys_str_mv AT latorreestivalisjosemanuel themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT robertsonhm themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT waldenkko themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT ruizj themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT goncalveslo themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT guarneriaa themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT lorenzomg themolecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT latorreestivalisjosemanuel molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT robertsonhm molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT waldenkko molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT ruizj molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT goncalveslo molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT guarneriaa molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
AT lorenzomg molecularsensorymachineryofachagasdiseasevectorexpressionchangesthroughimaginalmoultandsexuallydimorphicfeatures
bdutipo_str Repositorios
_version_ 1764820489326297091