Tailored polyelectrolyte thin film multilayers to modulate cell adhesion

The layer-by-layer assembly of polyelectrolyte multilayers (PEMs) from natural or synthetic polyelectrolytes constitutes a very versatile and simple strategy to modify surfaces and modulate cell behavior. PEMs assembled from natural polyelectrolytes are very appealing for biological and medical appl...

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Autores principales: Muzzio, Nicolás Eduardo, Pasquale, Miguel Ángel, Moya, Sergio E., Azzaroni, Omar
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Ciencias Exactas
Polyelectrolyte multilayers
Biocompatibility
Cell adhesion
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87697
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87697
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Ciencias Exactas
Polyelectrolyte multilayers
Biocompatibility
Cell adhesion
spellingShingle Química
Ciencias Exactas
Polyelectrolyte multilayers
Biocompatibility
Cell adhesion
Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Moya, Sergio E.
Azzaroni, Omar
Tailored polyelectrolyte thin film multilayers to modulate cell adhesion
topic_facet Química
Ciencias Exactas
Polyelectrolyte multilayers
Biocompatibility
Cell adhesion
description The layer-by-layer assembly of polyelectrolyte multilayers (PEMs) from natural or synthetic polyelectrolytes constitutes a very versatile and simple strategy to modify surfaces and modulate cell behavior. PEMs assembled from natural polyelectrolytes are very appealing for biological and medical applications due to their high biocompatibility. However, PEMs from natural polyelectrolytes display poor cell adhesion as they are soft materials with an elasticity modulus of a few kilopascal. In this report, the authors present results on the modulation of cell adhesion of different immortalized cell lines by PEMs. Two strategies are employed to vary cell adhesion: (1) a heterogeneous polyelectrolyte multilayer is assembled employing a rigid bottom block including a synthetic polyelectrolyte with a soft upper block of natural polyelectrolytes and (2) polyelectrolyte multilayers from natural polyelectrolytes are thermally annealed after assembly. The physicochemical characteristics of the PEMs change upon thermal treatment. Depending on the composition of the polyelectrolyte multilayer, cell adhesion may be enhanced or reduced. Based on the impact on PEM properties and cell adhesion caused by thermal annealing, a temperature gradient is applied to a PEM of poly-L-lysine/alginate to induce a spatial variation of PEM properties, resulting in a gradient in cell adhesion. The strategies shown here can be employed as simple alternatives to tailor PEM properties by means of fully biocompatible procedures.
format Articulo
Articulo
author Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Moya, Sergio E.
Azzaroni, Omar
author_facet Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Moya, Sergio E.
Azzaroni, Omar
author_sort Muzzio, Nicolás Eduardo
title Tailored polyelectrolyte thin film multilayers to modulate cell adhesion
title_short Tailored polyelectrolyte thin film multilayers to modulate cell adhesion
title_full Tailored polyelectrolyte thin film multilayers to modulate cell adhesion
title_fullStr Tailored polyelectrolyte thin film multilayers to modulate cell adhesion
title_full_unstemmed Tailored polyelectrolyte thin film multilayers to modulate cell adhesion
title_sort tailored polyelectrolyte thin film multilayers to modulate cell adhesion
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87697
work_keys_str_mv AT muzzionicolaseduardo tailoredpolyelectrolytethinfilmmultilayerstomodulatecelladhesion
AT pasqualemiguelangel tailoredpolyelectrolytethinfilmmultilayerstomodulatecelladhesion
AT moyasergioe tailoredpolyelectrolytethinfilmmultilayerstomodulatecelladhesion
AT azzaroniomar tailoredpolyelectrolytethinfilmmultilayerstomodulatecelladhesion
bdutipo_str Repositorios
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