Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies

In this study we developed an indirect ELISA to detect antibodies against Minute Virus of Mice (MVM) using an antigen produced from BHK-21 cells infected with a prototype strain of the virus. The optimal antigen concentration and serum dilutions were established. In order to analyze variability in t...

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Detalles Bibliográficos
Autores principales: Laborde, Juan Martín, Sguazza, Guillermo Hernán, Fuentealba, Nadia Analía, Corva, Santiago Gerardo, Carbone, Cecilia, Galosi, Cecilia Mónica
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
Diagnosis
ELISA
Minute Virus of Mice
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87688
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87688
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Diagnosis
ELISA
Minute Virus of Mice
spellingShingle Ciencias Veterinarias
Diagnosis
ELISA
Minute Virus of Mice
Laborde, Juan Martín
Sguazza, Guillermo Hernán
Fuentealba, Nadia Analía
Corva, Santiago Gerardo
Carbone, Cecilia
Galosi, Cecilia Mónica
Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies
topic_facet Ciencias Veterinarias
Diagnosis
ELISA
Minute Virus of Mice
description In this study we developed an indirect ELISA to detect antibodies against Minute Virus of Mice (MVM) using an antigen produced from BHK-21 cells infected with a prototype strain of the virus. The optimal antigen concentration and serum dilutions were established. In order to analyze variability in the laboratory, reproducibility and repeatability within and between plates were determined. Then, a panel of 460 sera from conventional facilities and previously classified as positive or negative by the indirect fluorescent antibody assay was analyzed. The cutoff value was determined by a receiver operating characteristic (ROC) curve. The results of the indirect ELISA were compared with those of the indirect fluorescent antibody assay. The ELISA assay showed 100% sensitivity and 99% specificity. ELISA is a useful tool to be developed in standard virology laboratories and can be used for screening animals faster than the traditional indirect fluorescent antibody assay.
format Articulo
Comunicacion
author Laborde, Juan Martín
Sguazza, Guillermo Hernán
Fuentealba, Nadia Analía
Corva, Santiago Gerardo
Carbone, Cecilia
Galosi, Cecilia Mónica
author_facet Laborde, Juan Martín
Sguazza, Guillermo Hernán
Fuentealba, Nadia Analía
Corva, Santiago Gerardo
Carbone, Cecilia
Galosi, Cecilia Mónica
author_sort Laborde, Juan Martín
title Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies
title_short Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies
title_full Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies
title_fullStr Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies
title_full_unstemmed Indirect ELISA (iELISA) for routine detection of antibodies against Minute Virus of Mice (MVM) in mice colonies
title_sort indirect elisa (ielisa) for routine detection of antibodies against minute virus of mice (mvm) in mice colonies
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87688
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