Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis

Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGF...

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Autores principales: García Mitacek, María Carla, Praderio, Romina Gisele, Stornelli, María Cecilia, Sota, Rodolfo Luzbel de la, Stornelli, María Alejandra
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
Endometritis
Domestic species
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87656
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87656
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Endometritis
Domestic species
spellingShingle Ciencias Veterinarias
Endometritis
Domestic species
García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
topic_facet Ciencias Veterinarias
Endometritis
Domestic species
description Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGFS) in the endometrium of bitches with or without endometritis. Thirty mixed breed bitches in dioestrus, aged between 1 and 5 years, and weighing between 10 and 30 kg were used. After ovariohysterectomy (OVX), uterine biopsy samples were collected from the middle part of both horns. Then, endometrial epithelium was collected using the cytobrush method and mRNA analysis was performed by real-time RT-PCR. Data were analysed with Kruskal–Wallis anova using the sas® software. Uterine condition was identified by endometrial biopsies (normal endometria [n = 11; NE], acute endometritis [n = 10; AE] and chronic endometritis [n = 9; CE]). The COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in bitches with and without endometritis was similar. Except for PGFS/AKR1C3, gene transcription of COX2 and PTGES-1 was significantly increased in AE compared with CE. In addition, COX2 gene transcription was significantly increased in AE compared with NE. In contrast, no differences were found for COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in the samples of NE compared with CE.
format Articulo
Articulo
author García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author_facet García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author_sort García Mitacek, María Carla
title Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_short Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_full Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_fullStr Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_full_unstemmed Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_sort prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87656
work_keys_str_mv AT garciamitacekmariacarla prostaglandinsynthesisenzymesgenetranscriptioninbitcheswithendometritis
AT praderiorominagisele prostaglandinsynthesisenzymesgenetranscriptioninbitcheswithendometritis
AT stornellimariacecilia prostaglandinsynthesisenzymesgenetranscriptioninbitcheswithendometritis
AT sotarodolfoluzbeldela prostaglandinsynthesisenzymesgenetranscriptioninbitcheswithendometritis
AT stornellimariaalejandra prostaglandinsynthesisenzymesgenetranscriptioninbitcheswithendometritis
bdutipo_str Repositorios
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