<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)

The pampean region in the Buenos Aires province of Argentina is a major horticultural area. Biological pesticides to control pest insects available in the market are scarce in this country and nonexistent based on entomonematodes. In this paper, we characterized a native entomopathogenic nematode is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eliceche, Daiana Pamela, Belaich, Mariano Nicolás, Ghiringhelli, Daniel, Achinelly, María Fernanda
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Naturales Biological control Characterization Entomopathogenic nematodes Heterorhabditidae
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/87616
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-87616
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Biological control Characterization Entomopathogenic nematodes Heterorhabditidae
spellingShingle	Ciencias Naturales Biological control Characterization Entomopathogenic nematodes Heterorhabditidae Eliceche, Daiana Pamela Belaich, Mariano Nicolás Ghiringhelli, Daniel Achinelly, María Fernanda <i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)
topic_facet	Ciencias Naturales Biological control Characterization Entomopathogenic nematodes Heterorhabditidae
description	The pampean region in the Buenos Aires province of Argentina is a major horticultural area. Biological pesticides to control pest insects available in the market are scarce in this country and nonexistent based on entomonematodes. In this paper, we characterized a native entomopathogenic nematode isolated from soil in the pampas, and evaluated its infectivity and reproductive capacity in the strawberry sap beetle <i>Lobiopa insularis</i>. Morphological and molecular-genetic analysis determined this isolate to be a nematode of <i>Heterorhabditis bacteriophora</i>, thereafter designated as the VEli strain. A 901-bp genomic-DNA fragment was isolated (GenBank Accesion No KJ575524). The new strain was pathogenic against <i>L. insularis</i>. In 200 ml containers with autoclaved clay soil (100 ml), at concentrations of 1,000; 5,000 and 10, 000 IJs, mortality in larvae ranged from 25 to 77 %. At a concentration of 10,000 IJs, infectivity was higher in larvae and pupae (77 ± 20% and 64 ± 25 %, respectively) than in adults (2.2 ± 1.1 %). The lethal concentration required to kill 50 and 90 % of the larvae (LC<sub>50</sub> and LC<sub>90</sub>) over 10 days was 2,840 and 23,743 IJs. Differences were observed in the parasitism levels and emergence time of IJs between concentrations and stages, although not for production. <i>Heterorhabditis bacteriophora</i> strain VEli is the first entomopathogenic nematode found to infect and kill <i>L. insularis</i>, capable of completing its life cycle in all strawberry sap beetle stages. This availability would enable the establishment and subsequent dispersal of the strain in strawberry fields. Finally, this report constitutes the first presentation of data on the morphological, morphometric, and molecular-genetic characterization of a native entomopathogenic nematode strain from the Buenos Aires province, Argentina.
format	Articulo Articulo
author	Eliceche, Daiana Pamela Belaich, Mariano Nicolás Ghiringhelli, Daniel Achinelly, María Fernanda
author_facet	Eliceche, Daiana Pamela Belaich, Mariano Nicolás Ghiringhelli, Daniel Achinelly, María Fernanda
author_sort	Eliceche, Daiana Pamela
title	<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)
title_short	<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)
title_full	<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)
title_fullStr	<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)
title_full_unstemmed	<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)
title_sort	<i>heterorhabditis bacteriophora</i> pampean-strain veli (nematoda): identification and pathogenicity against the strawberry pest <i>lobiopa insularis</i> (coleoptera: nitidulidae)
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/87616
work_keys_str_mv	AT elicechedaianapamela iheterorhabditisbacteriophoraipampeanstrainvelinematodaidentificationandpathogenicityagainstthestrawberrypestilobiopainsularisicoleopteranitidulidae AT belaichmarianonicolas iheterorhabditisbacteriophoraipampeanstrainvelinematodaidentificationandpathogenicityagainstthestrawberrypestilobiopainsularisicoleopteranitidulidae AT ghiringhellidaniel iheterorhabditisbacteriophoraipampeanstrainvelinematodaidentificationandpathogenicityagainstthestrawberrypestilobiopainsularisicoleopteranitidulidae AT achinellymariafernanda iheterorhabditisbacteriophoraipampeanstrainvelinematodaidentificationandpathogenicityagainstthestrawberrypestilobiopainsularisicoleopteranitidulidae AT elicechedaianapamela iheterorhabditisbacteriophoraicepavelinematodaidentificacionypatogeniasobreilobiopainsularisicoleopteranitidulidaeplagadelafresa AT belaichmarianonicolas iheterorhabditisbacteriophoraicepavelinematodaidentificacionypatogeniasobreilobiopainsularisicoleopteranitidulidaeplagadelafresa AT ghiringhellidaniel iheterorhabditisbacteriophoraicepavelinematodaidentificacionypatogeniasobreilobiopainsularisicoleopteranitidulidaeplagadelafresa AT achinellymariafernanda iheterorhabditisbacteriophoraicepavelinematodaidentificacionypatogeniasobreilobiopainsularisicoleopteranitidulidaeplagadelafresa
bdutipo_str	Repositorios
_version_	1764820489220390914

<i>Heterorhabditis bacteriophora</i> pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest <i>Lobiopa insularis</i> (Coleoptera: Nitidulidae)

Ejemplares similares