Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications

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We present the construction of layer-by-layer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect transistors (rGO FETs) for the detection of urea. This versatile biosensor platform simultaneously exploits the pH dependency of liquid-gated graphene-based tr...

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Detalles Bibliográficos
Autores principales: Piccinini, Esteban, Bliem, Christina, Reiner Rozman, Ciril, Battaglini, Fernando, Azzaroni, Omar, Knoll, Wolfgang
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Biosensors
Chemical sensors
Field-effect transistors
Graphene
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87598
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87598
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biosensors
Chemical sensors
Field-effect transistors
Graphene
spellingShingle Ciencias Exactas
Biosensors
Chemical sensors
Field-effect transistors
Graphene
Piccinini, Esteban
Bliem, Christina
Reiner Rozman, Ciril
Battaglini, Fernando
Azzaroni, Omar
Knoll, Wolfgang
Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
topic_facet Ciencias Exactas
Biosensors
Chemical sensors
Field-effect transistors
Graphene
description We present the construction of layer-by-layer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect transistors (rGO FETs) for the detection of urea. This versatile biosensor platform simultaneously exploits the pH dependency of liquid-gated graphene-based transistors and the change in the local pH produced by the catalyzed hydrolysis of urea. The use of an interdigitated microchannel resulted in transistors displaying low noise, high pH sensitivity (20.3 µA/pH) and transconductance values up to 800 µS. The modification of rGO FETs with a weak polyelectrolyte improved the pH response because of its transducing properties by electrostatic gating effects. In the presence of urea, the urease-modified rGO FETs showed a shift in the Dirac point due to the change in the local pH close to the graphene surface. Markedly, these devices operated at very low voltages (less than 500 mV) and were able to monitor urea in the range of 1–1000 µm, with a limit of detection (LOD) down to 1 µm, fast response and good long-term stability. The urea-response of the transistors was enhanced by increasing the number of bilayers due to the increment of the enzyme surface coverage onto the channel. Moreover, quantification of the heavy metal Cu<sup>2+</sup>(with a LOD down to 10 nM) was performed in aqueous solution by taking advantage of the urease specific inhibition.
format Articulo
Articulo
author Piccinini, Esteban
Bliem, Christina
Reiner Rozman, Ciril
Battaglini, Fernando
Azzaroni, Omar
Knoll, Wolfgang
author_facet Piccinini, Esteban
Bliem, Christina
Reiner Rozman, Ciril
Battaglini, Fernando
Azzaroni, Omar
Knoll, Wolfgang
author_sort Piccinini, Esteban
title Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
title_short Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
title_full Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
title_fullStr Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
title_full_unstemmed Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
title_sort enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87598
work_keys_str_mv AT piccininiesteban enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications
AT bliemchristina enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications
AT reinerrozmanciril enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications
AT battaglinifernando enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications
AT azzaroniomar enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications
AT knollwolfgang enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications
bdutipo_str Repositorios
_version_ 1764820489201516546

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