Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina

In Argentina, glyphosate resistance was reported in a <i>Lolium perenne</i> population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses...

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Detalles Bibliográficos
Autores principales: Yanniccari, Marcos, Gómez Lobato, María Eugenia, Istilart, Carolina, Natalucci, Claudia Luisa, Giménez, Daniel Oscar, Castro, Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87593
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87593
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
spellingShingle Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
Yanniccari, Marcos
Gómez Lobato, María Eugenia
Istilart, Carolina
Natalucci, Claudia Luisa
Giménez, Daniel Oscar
Castro, Ana María
Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
topic_facet Ciencias Agrarias
Ciencias Exactas
EPSPS activity
EPSPS overexpression
Glyphosate resistance
Perennial ryegrass
Shikimate assay
description In Argentina, glyphosate resistance was reported in a <i>Lolium perenne</i> population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated <i>in vitro</i> showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
format Articulo
Articulo
author Yanniccari, Marcos
Gómez Lobato, María Eugenia
Istilart, Carolina
Natalucci, Claudia Luisa
Giménez, Daniel Oscar
Castro, Ana María
author_facet Yanniccari, Marcos
Gómez Lobato, María Eugenia
Istilart, Carolina
Natalucci, Claudia Luisa
Giménez, Daniel Oscar
Castro, Ana María
author_sort Yanniccari, Marcos
title Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_short Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_full Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_fullStr Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_full_unstemmed Mechanism of resistance to glyphosate in <i>Lolium perenne</i> from Argentina
title_sort mechanism of resistance to glyphosate in <i>lolium perenne</i> from argentina
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87593
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