Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus

Mitochondrial dysfunction is a common hallmark in aging. In the female, reproductive senescence is characterized by loss of ovarian hormones, many of whose neuroprotective effects converge upon mitochondria. The functional integrity of mitochondria is dependent on membrane fatty acid and phospholipi...

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Autores principales:	Zárate, Sandra, Astiz, Mariana, Magnani, Natalia, Imsen, Mercedes, Merino, Florencia, Álvarez, Silvia, Reinés, Analía, Seilicovich, Adriana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Médicas Ciencias Exactas Aging Cardiolipin Hippocampus Mitochondria Ovariectomy PUFAs
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/87565
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-87565
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Ciencias Exactas Aging Cardiolipin Hippocampus Mitochondria Ovariectomy PUFAs
spellingShingle	Ciencias Médicas Ciencias Exactas Aging Cardiolipin Hippocampus Mitochondria Ovariectomy PUFAs Zárate, Sandra Astiz, Mariana Magnani, Natalia Imsen, Mercedes Merino, Florencia Álvarez, Silvia Reinés, Analía Seilicovich, Adriana Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
topic_facet	Ciencias Médicas Ciencias Exactas Aging Cardiolipin Hippocampus Mitochondria Ovariectomy PUFAs
description	Mitochondrial dysfunction is a common hallmark in aging. In the female, reproductive senescence is characterized by loss of ovarian hormones, many of whose neuroprotective effects converge upon mitochondria. The functional integrity of mitochondria is dependent on membrane fatty acid and phospholipid composition, which are also affected during aging. The effect of long-term ovarian hormone deprivation upon mitochondrial function and its putative association with changes in mitochondrial membrane lipid profile in the hippocampus, an area primarily affected during aging and highly responsive to ovarian hormones, is unknown. To this aim, Wistar adult female rats were ovariectomized or sham-operated. Twelve weeks later, different parameters of mitochondrial function (O2 uptake, ATP production, membrane potential and respiratory complex activities) as well as membrane phospholipid content and composition were evaluated in hippocampal mitochondria. Chronic ovariectomy reduced mitochondrial O2 uptake and ATP production rates and induced membrane depolarization during active respiration without altering the activity of respiratory complexes. Mitochondrial membrane lipid profile showed no changes in cholesterol levels but higher levels of unsaturated fatty acids and a higher peroxidizability index in mitochondria from ovariectomized rats. Interestingly, ovariectomy also reduced cardiolipin content and altered cardiolipin fatty acid profile leading to a lower peroxidizability index. In conclusion, chronic ovarian hormone deprivation induces mitochondrial dysfunction and changes in the mitochondrial membrane lipid profile comparable to an aging phenotype. Our study provides insights into ovarian hormone loss-induced early lipidomic changes with bioenergetic deficits in the hippocampus that may contribute to the increased risk of Alzheimer's disease and other age-associated disorders observed in postmenopause.
format	Articulo Articulo
author	Zárate, Sandra Astiz, Mariana Magnani, Natalia Imsen, Mercedes Merino, Florencia Álvarez, Silvia Reinés, Analía Seilicovich, Adriana
author_facet	Zárate, Sandra Astiz, Mariana Magnani, Natalia Imsen, Mercedes Merino, Florencia Álvarez, Silvia Reinés, Analía Seilicovich, Adriana
author_sort	Zárate, Sandra
title	Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
title_short	Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
title_full	Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
title_fullStr	Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
title_full_unstemmed	Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
title_sort	hormone deprivation alters mitochondrial function and lipid profile in the hippocampus
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/87565
work_keys_str_mv	AT zaratesandra hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT astizmariana hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT magnaninatalia hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT imsenmercedes hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT merinoflorencia hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT alvarezsilvia hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT reinesanalia hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus AT seilicovichadriana hormonedeprivationaltersmitochondrialfunctionandlipidprofileinthehippocampus
bdutipo_str	Repositorios
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Hormone deprivation alters mitochondrial function and lipid profile in the hippocampus

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