Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance

The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the "protein corona" around the nano...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Di Ianni, Mauricio Emiliano, Islan, Germán Abel, Chain, Cecilia Yamil, Castro, Guillermo Raúl, Talevi, Alan, Vela, María Elena
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Química
Body fluids
Medical nanotechnology
Nanoparticles
Proteins
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87487
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87487
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Body fluids
Medical nanotechnology
Nanoparticles
Proteins
spellingShingle Ciencias Exactas
Química
Body fluids
Medical nanotechnology
Nanoparticles
Proteins
Di Ianni, Mauricio Emiliano
Islan, Germán Abel
Chain, Cecilia Yamil
Castro, Guillermo Raúl
Talevi, Alan
Vela, María Elena
Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
topic_facet Ciencias Exactas
Química
Body fluids
Medical nanotechnology
Nanoparticles
Proteins
description The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the "protein corona" around the nanoparticle, that is, a complex biomolecular layer formed when the nanovehicle is exposed to biological fluids. The chemical nature of the protein corona determines the biological identity of the nanoparticle and influences, among others, the recognition of the nanocarrier by the mononuclear phagocytic system and, thus, its clearance from the blood. Recent works suggest that Surface Plasmon Resonance (SPR), extensively employed for the analysis of biomolecular interactions, can shed light on the formation of the protein corona and its interaction with the surroundings. The synthesis and characterization of solid lipid nanoparticles (SLN) coated with polymers of different chemical nature (e.g., polyvinyl alcohol, chitosans) are reported. The proof-of-concept for the use of SPR technique in characterizing protein-nanoparticle interactions of surface-immobilized proteins (immunoglobulin G and bovine serum albumin, both involved in the formation of the corona) subjected to flowing SLN is demonstrated for non-chitosan-coated nanoparticles. All assayed nanosystems show more preference for IgG than for BSA, such preference being more pronounced in the case of polyvinyl-alcohol-coated SLN.
format Articulo
Articulo
author Di Ianni, Mauricio Emiliano
Islan, Germán Abel
Chain, Cecilia Yamil
Castro, Guillermo Raúl
Talevi, Alan
Vela, María Elena
author_facet Di Ianni, Mauricio Emiliano
Islan, Germán Abel
Chain, Cecilia Yamil
Castro, Guillermo Raúl
Talevi, Alan
Vela, María Elena
author_sort Di Ianni, Mauricio Emiliano
title Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
title_short Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
title_full Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
title_fullStr Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
title_full_unstemmed Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
title_sort interaction of solid lipid nanoparticles and specific proteins of the corona studied by surface plasmon resonance
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87487
work_keys_str_mv AT diiannimauricioemiliano interactionofsolidlipidnanoparticlesandspecificproteinsofthecoronastudiedbysurfaceplasmonresonance
AT islangermanabel interactionofsolidlipidnanoparticlesandspecificproteinsofthecoronastudiedbysurfaceplasmonresonance
AT chainceciliayamil interactionofsolidlipidnanoparticlesandspecificproteinsofthecoronastudiedbysurfaceplasmonresonance
AT castroguillermoraul interactionofsolidlipidnanoparticlesandspecificproteinsofthecoronastudiedbysurfaceplasmonresonance
AT talevialan interactionofsolidlipidnanoparticlesandspecificproteinsofthecoronastudiedbysurfaceplasmonresonance
AT velamariaelena interactionofsolidlipidnanoparticlesandspecificproteinsofthecoronastudiedbysurfaceplasmonresonance
bdutipo_str Repositorios
_version_ 1764820490111680515

Ejemplares similares