Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil

In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OT...

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Detalles Bibliográficos
Autores principales: Calderoli, Priscila Anabel, Collavino, Mónica Mariana, Behrends Kraemer, Filipe, Morrás, Héctor J. M., Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87448
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87448
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
spellingShingle Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
Calderoli, Priscila Anabel
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Orlando Mario
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
topic_facet Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
description In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
format Articulo
Articulo
author Calderoli, Priscila Anabel
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Orlando Mario
author_facet Calderoli, Priscila Anabel
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Orlando Mario
author_sort Calderoli, Priscila Anabel
title Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_short Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_full Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_fullStr Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_full_unstemmed Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_sort analysis of nifh-rna reveals phylotypes related to geobacter and cyanobacteria as important functional components of the n<sub>2</sub>-fixing community depending on depth and agricultural use of soil
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87448
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