Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports

The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-Aminopropyl-Triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h we...

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Detalles Bibliográficos
Autores principales: Vargas, Annie Y., Rojas, Hugo A., Romanelli, Gustavo Pablo, Martínez Zambrano, José Jobanny
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Biginelli/Hantzsch reaction
magnetic supports
urease immobilized
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87296
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87296
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biginelli/Hantzsch reaction
magnetic supports
urease immobilized
spellingShingle Química
Biginelli/Hantzsch reaction
magnetic supports
urease immobilized
Vargas, Annie Y.
Rojas, Hugo A.
Romanelli, Gustavo Pablo
Martínez Zambrano, José Jobanny
Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
topic_facet Química
Biginelli/Hantzsch reaction
magnetic supports
urease immobilized
description The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-Aminopropyl-Triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.
format Articulo
Articulo
author Vargas, Annie Y.
Rojas, Hugo A.
Romanelli, Gustavo Pablo
Martínez Zambrano, José Jobanny
author_facet Vargas, Annie Y.
Rojas, Hugo A.
Romanelli, Gustavo Pablo
Martínez Zambrano, José Jobanny
author_sort Vargas, Annie Y.
title Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_short Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_full Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_fullStr Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_full_unstemmed Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_sort synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87296
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AT romanelligustavopablo synthesisof14dihydropyrimidineswithimmobilizedureaseeffectofmethodimmobilizationonmagneticsupports
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