Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain

The preprophase band of microtubules performs the crucial function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast is attracted during cytokin...

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Detalles Bibliográficos
Autores principales: Buschmann, H., Dols, J., Kopischke, S., Peña, Eduardo José, Andrade Navarro, M. A., Heinlein, M., Szymanski, D. B., Zachgo, S., Doonan, J. H., Lloyd, C. W.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
AIR9
Arabidopsis
Cell division
Kinesin
MyTH4-FERM
Preprophase band
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87252
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87252
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
AIR9
Arabidopsis
Cell division
Kinesin
MyTH4-FERM
Preprophase band
spellingShingle Ciencias Exactas
AIR9
Arabidopsis
Cell division
Kinesin
MyTH4-FERM
Preprophase band
Buschmann, H.
Dols, J.
Kopischke, S.
Peña, Eduardo José
Andrade Navarro, M. A.
Heinlein, M.
Szymanski, D. B.
Zachgo, S.
Doonan, J. H.
Lloyd, C. W.
Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
topic_facet Ciencias Exactas
AIR9
Arabidopsis
Cell division
Kinesin
MyTH4-FERM
Preprophase band
description The preprophase band of microtubules performs the crucial function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast is attracted during cytokinesis. Proteins have been discovered that are part of the molecular memory but little is known about how they contribute to phragmoplast guidance. Previously, we found that the microtubule-associated protein AIR9 is found in the cortical division zone at preprophase and returns during cell plate insertion but is absent from the cortex during the intervening mitosis. To identify new components of the preprophase memory, we searched for proteins that interact with AIR9. We detected the kinesin-like calmodulin-binding protein, KCBP, which can be visualized at the predicted cortical site throughout division. A truncation study of KCBP indicates that its MyTH4-FERM domain is required for linking the motor domain to the cortex. These results suggest a mechanism by which minus-end-directed KCBP helps guide the centrifugally expanding phragmoplast to the cortical division site.
format Articulo
Articulo
author Buschmann, H.
Dols, J.
Kopischke, S.
Peña, Eduardo José
Andrade Navarro, M. A.
Heinlein, M.
Szymanski, D. B.
Zachgo, S.
Doonan, J. H.
Lloyd, C. W.
author_facet Buschmann, H.
Dols, J.
Kopischke, S.
Peña, Eduardo José
Andrade Navarro, M. A.
Heinlein, M.
Szymanski, D. B.
Zachgo, S.
Doonan, J. H.
Lloyd, C. W.
author_sort Buschmann, H.
title Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
title_short Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
title_full Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
title_fullStr Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
title_full_unstemmed Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
title_sort arabidopsis kcbp interacts with air9 but stays in the cortical division zone throughout mitosis via its myth4-ferm domain
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/87252
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