Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study

In mammalian cells, <i>de novo</i> glycerolipid synthesis begins with the acylation of glycerol-3-phosphate, catalyzed by glycerol-3-phosphate acyltransferases (GPAT). GPAT2 is a mitochondrial isoform primarily expressed in testis under physiological conditions, and overexpressed in seve...

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Detalles Bibliográficos
Autores principales: Cattaneo, Elizabeth Renee, Prieto, Eduardo Daniel, García Fabiani, María Belén, Montanaro, Mauro Aldo, Guillou, Herve, González Baró, María del Rosario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Ciencias Médicas
Glycerol-3-phosphate
microscopy study
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87225
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87225
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ciencias Médicas
Glycerol-3-phosphate
microscopy study
spellingShingle Ciencias Exactas
Ciencias Médicas
Glycerol-3-phosphate
microscopy study
Cattaneo, Elizabeth Renee
Prieto, Eduardo Daniel
García Fabiani, María Belén
Montanaro, Mauro Aldo
Guillou, Herve
González Baró, María del Rosario
Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study
topic_facet Ciencias Exactas
Ciencias Médicas
Glycerol-3-phosphate
microscopy study
description In mammalian cells, <i>de novo</i> glycerolipid synthesis begins with the acylation of glycerol-3-phosphate, catalyzed by glycerol-3-phosphate acyltransferases (GPAT). GPAT2 is a mitochondrial isoform primarily expressed in testis under physiological conditions, and overexpressed in several types of cancers and cancer-derived human cell lines where its expression contributes to the tumor phenotype. Using gene silencing and atomic force microscopy, we studied the correlation between GPAT2 expression and cell surface topography, roughness and membrane permeability in MDA-MB-231 cells. In addition, we analyzed the glycerolipid composition by gas-liquid chromatography. GPAT2 expression altered the arachidonic acid content in glycerolipids, and the lack of GPAT2 seems to be partially compensated by the overexpression of another arachidonic-acid-metabolizing enzyme, AGPAT11. GPAT2 expressing cells exhibited a rougher topography and less membrane damage than GPAT2 silenced cells. Pore-like structures were present only in GPAT2 subexpressing cells, correlating with higher membrane damage evidenced by lactate dehydrogenase release. These GPAT2-induced changes are consistent with its proposed function as a tumor-promoting gene, and might be used as a phenotypic differentiation marker. AFM provides the basis for the identification and quantification of those changes, and demonstrates the utility of this technique in the study of cancer cell biology.
format Articulo
Articulo
author Cattaneo, Elizabeth Renee
Prieto, Eduardo Daniel
García Fabiani, María Belén
Montanaro, Mauro Aldo
Guillou, Herve
González Baró, María del Rosario
author_facet Cattaneo, Elizabeth Renee
Prieto, Eduardo Daniel
García Fabiani, María Belén
Montanaro, Mauro Aldo
Guillou, Herve
González Baró, María del Rosario
author_sort Cattaneo, Elizabeth Renee
title Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study
title_short Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study
title_full Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study
title_fullStr Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study
title_full_unstemmed Glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: An atomic force microscopy study
title_sort glycerol-3-phosphate acyltransferase 2 expression modulates cell roughness and membrane permeability: an atomic force microscopy study
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87225
work_keys_str_mv AT cattaneoelizabethrenee glycerol3phosphateacyltransferase2expressionmodulatescellroughnessandmembranepermeabilityanatomicforcemicroscopystudy
AT prietoeduardodaniel glycerol3phosphateacyltransferase2expressionmodulatescellroughnessandmembranepermeabilityanatomicforcemicroscopystudy
AT garciafabianimariabelen glycerol3phosphateacyltransferase2expressionmodulatescellroughnessandmembranepermeabilityanatomicforcemicroscopystudy
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AT guillouherve glycerol3phosphateacyltransferase2expressionmodulatescellroughnessandmembranepermeabilityanatomicforcemicroscopystudy
AT gonzalezbaromariadelrosario glycerol3phosphateacyltransferase2expressionmodulatescellroughnessandmembranepermeabilityanatomicforcemicroscopystudy
bdutipo_str Repositorios
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