Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)

The freshwater snail <i>Galba cubensis</i> (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of <i>Fasciola hepatica</i> - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied....

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Autores principales: Lounnas, Manon, Vázquez, Antonio A., Alda, María del Pilar, Sartori, Kevin, Pointier, Jean Pierre, David, Patrice, Hurtrez Boussès, Sylvie
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Naturales
Galba cubensis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87185
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87185
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Galba cubensis
spellingShingle Ciencias Naturales
Galba cubensis
Lounnas, Manon
Vázquez, Antonio A.
Alda, María del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
topic_facet Ciencias Naturales
Galba cubensis
description The freshwater snail <i>Galba cubensis</i> (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of <i>Fasciola hepatica</i> - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in <i>G. cubensis</i> to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of <i>G. cubensis</i> from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: <i>G. truncatula</i>, <i>G. viator</i> and <i>G. neotropica</i>. We found that <i>G. cubensis</i> has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in <i>G. truncatula</i> and <i>G. viator</i>, respectively, and that all 15 loci amplified in <i>G. neotropica</i>. This last finding suggests a close relatedness between <i>G. cubensis</i> and <i>G. neotropica</i>, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
format Articulo
Articulo
author Lounnas, Manon
Vázquez, Antonio A.
Alda, María del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author_facet Lounnas, Manon
Vázquez, Antonio A.
Alda, María del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author_sort Lounnas, Manon
title Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_short Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_full Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_fullStr Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_full_unstemmed Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_sort isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>galba cubensis</i> (lymnaeidae)
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87185
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