Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model
Lactate has long been considered as a metabolic by-product of cells. Recently, this view has been changed by the observation that lactate can act as a signaling molecule and regulates critical functions of the immune system. We previously identified lactate as the component responsible for the modul...
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| Autores principales: | , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86913 |
| Aporte de: |
| id |
I19-R120-10915-86913 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Flagellin Immunomodulation Innate immunity Lactate TNBS-induced colitis |
| spellingShingle |
Ciencias Exactas Flagellin Immunomodulation Innate immunity Lactate TNBS-induced colitis Iraporda, Carolina Romanin, David Emmanuel Bengoa, Ana Agustina Errea, Agustina Juliana Cayet, D. Foligné, B. Sirard, J.-C. Garrote, Graciela Liliana Abraham, Analía Graciela Rumbo, Martín Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model |
| topic_facet |
Ciencias Exactas Flagellin Immunomodulation Innate immunity Lactate TNBS-induced colitis |
| description |
Lactate has long been considered as a metabolic by-product of cells. Recently, this view has been changed by the observation that lactate can act as a signaling molecule and regulates critical functions of the immune system. We previously identified lactate as the component responsible for the modulation of innate immune epithelial response of fermented milk supernatants in vitro. We have also shown that lactate downregulates proinflammatory responses of macrophages and dendritic cells. So far, in vivo effects of lactate on intestinal inflammation have not been reported. We evaluated the effect of intrarectal administration of lactate in a murine model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). The increase in lactate concentration in colon promoted protective effects against TNBS-induced colitis preventing histopathological damage, as well as bacterial translocation and rise of IL-6 levels in serum. Using intestinal epithelial reporter cells, we found that flagellin treatment induced reporter gene expression, which was abrogated by lactate treatment as well as by glycolysis inhibitors. Furthermore, lactate treatment modulated glucose uptake, indicating that high levels of extracellular lactate can impair metabolic reprograming induced by proinflammatory activation. These results suggest that lactate could be a potential beneficial microbiota metabolite and may constitute an overlooked effector with modulatory properties. |
| format |
Articulo Articulo |
| author |
Iraporda, Carolina Romanin, David Emmanuel Bengoa, Ana Agustina Errea, Agustina Juliana Cayet, D. Foligné, B. Sirard, J.-C. Garrote, Graciela Liliana Abraham, Analía Graciela Rumbo, Martín |
| author_facet |
Iraporda, Carolina Romanin, David Emmanuel Bengoa, Ana Agustina Errea, Agustina Juliana Cayet, D. Foligné, B. Sirard, J.-C. Garrote, Graciela Liliana Abraham, Analía Graciela Rumbo, Martín |
| author_sort |
Iraporda, Carolina |
| title |
Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model |
| title_short |
Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model |
| title_full |
Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model |
| title_fullStr |
Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model |
| title_full_unstemmed |
Local treatment with lactate prevents intestinal inflammation in the TNBS-induced colitis model |
| title_sort |
local treatment with lactate prevents intestinal inflammation in the tnbs-induced colitis model |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/86913 |
| work_keys_str_mv |
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Repositorios |
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1764820489445834755 |