Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing

The effect of bioaugmentation with <i>Sphingobium</i> sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that...

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Autores principales: Festa, Sabrina, Macchi, Marianela, Cortés, Federico, Morelli, Irma Susana, Coppotelli, Bibiana Marina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86746
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86746
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
spellingShingle Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
Festa, Sabrina
Macchi, Marianela
Cortés, Federico
Morelli, Irma Susana
Coppotelli, Bibiana Marina
Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
topic_facet Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
description The effect of bioaugmentation with <i>Sphingobium</i> sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on <i>Actinomycetales</i> and <i>Sphingomonadales</i> orders, while inoculation caused a relative decline of <i>Actinomycetales</i>. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of <i>Sphingomonadales</i>, <i>Burkholderiales</i> and <i>Rhizobiales</i> orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.
format Articulo
Articulo
author Festa, Sabrina
Macchi, Marianela
Cortés, Federico
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author_facet Festa, Sabrina
Macchi, Marianela
Cortés, Federico
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author_sort Festa, Sabrina
title Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_short Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_full Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_fullStr Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_full_unstemmed Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_sort monitoring the impact of bioaugmentation with a pah-degrading strain on different soil microbiomes using pyrosequencing
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86746
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