Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eigh...
| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2015
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86718 |
| Aporte de: |
| id |
I19-R120-10915-86718 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Fusarium graminearum Fusarium Head Blight Inter-simple sequence repeat Intergenic spacer-restriction fragment length polymorphism Wheat |
| spellingShingle |
Ciencias Exactas Fusarium graminearum Fusarium Head Blight Inter-simple sequence repeat Intergenic spacer-restriction fragment length polymorphism Wheat Consolo, Verónica Fabiana Ortega, Leonel Maximiliano Salerno, Graciela Lidia Astoreca, Andrea Luciana Alconada Magliano, Teresa María Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina |
| topic_facet |
Ciencias Exactas Fusarium graminearum Fusarium Head Blight Inter-simple sequence repeat Intergenic spacer-restriction fragment length polymorphism Wheat |
| description |
Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 47 polymorphic bands. Both markers showed genetic variability among the analyzed isolates; however, IGS-RFLP was more efficient than ISSR, showing a higher polymorphic average (59.91%) than the latter (44.11%). The averages of polymorphic information content (PIC) were 0.211 and 0.129, respectively. Twenty haplotypes were identified by IGS-RFLP and 15 haplotypes by ISSR. Genotype clustering within dendrograms was different for both types of markers. The genetic groups obtained by IGS-RFLP showed a partial association to geographic origin. This is the first report on genetic variability of <i>F. graminearum</i> isolates from wheat in Argentina using IGS-RFLP and ISSR markers. |
| format |
Articulo Articulo |
| author |
Consolo, Verónica Fabiana Ortega, Leonel Maximiliano Salerno, Graciela Lidia Astoreca, Andrea Luciana Alconada Magliano, Teresa María |
| author_facet |
Consolo, Verónica Fabiana Ortega, Leonel Maximiliano Salerno, Graciela Lidia Astoreca, Andrea Luciana Alconada Magliano, Teresa María |
| author_sort |
Consolo, Verónica Fabiana |
| title |
Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina |
| title_short |
Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina |
| title_full |
Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina |
| title_fullStr |
Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina |
| title_full_unstemmed |
Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina |
| title_sort |
genetic diversity of <i>fusarium graminearum sensu lato</i> isolates from wheat associated with fusarium head blight in diverse geographic locations of argentina |
| publishDate |
2015 |
| url |
http://sedici.unlp.edu.ar/handle/10915/86718 |
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Repositorios |
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