Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows

The expression of cytoskeletal proteins was evaluated immunohistochemically in 36 normal ovaries sampled from 18 sows and 44 cystic ovaries sampled from of 22 sows, was evaluated. All sows had history of reproductive problems, such as infertility or subfertility. The immunohistochemically stained ar...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Sant'Ana, Fabiano J.F., Reis Junior, Janildo L., Araújo, Rosélia L.S., Gimeno, Eduardo Juan, Ortega, Hugo H.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86660
Aporte de:
id I19-R120-10915-86660
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Cytoskeletal proteins
Ovarian cysts
Ovary
Swine
Cistos ovarianos
Ovário
Proteínas do citoesqueleto
Porca matriz
spellingShingle Ciencias Veterinarias
Cytoskeletal proteins
Ovarian cysts
Ovary
Swine
Cistos ovarianos
Ovário
Proteínas do citoesqueleto
Porca matriz
Sant'Ana, Fabiano J.F.
Reis Junior, Janildo L.
Araújo, Rosélia L.S.
Gimeno, Eduardo Juan
Ortega, Hugo H.
Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
topic_facet Ciencias Veterinarias
Cytoskeletal proteins
Ovarian cysts
Ovary
Swine
Cistos ovarianos
Ovário
Proteínas do citoesqueleto
Porca matriz
description The expression of cytoskeletal proteins was evaluated immunohistochemically in 36 normal ovaries sampled from 18 sows and 44 cystic ovaries sampled from of 22 sows, was evaluated. All sows had history of reproductive problems, such as infertility or subfertility. The immunohistochemically stained area (IHCSA) was quantified through image analysis to evaluate the expression of these proteins in the follicular wall of secondary, tertiary, and cystic follicles. Cytokeratins (CK) immunoreactivity was strong in the granulosa cell layer (GC) and mild in the theca interna (TI) and externa (TE) of the normal follicles. There was severe reduction of the reaction to CK in the GC in the cystic follicles, mainly in the luteinized cysts. The immunoreactivity for vimentin was higher in the GC from normal and cystic follicles in contrast with the other follicular structures. In the luteinized cysts, the IHCSA for vimentin was significantly higher in TI and in both observed cysts, the labeling was more accentuated in TE. Immunohistochemical detection of desmin and α-SMA was restricted to the TE, without differences between the normal and cystic follicles. The results of the current study show that the development of ovarian cysts in sows is associated to changes in the expression of the cytoskeletal proteins CK and vimentin.
format Articulo
Articulo
author Sant'Ana, Fabiano J.F.
Reis Junior, Janildo L.
Araújo, Rosélia L.S.
Gimeno, Eduardo Juan
Ortega, Hugo H.
author_facet Sant'Ana, Fabiano J.F.
Reis Junior, Janildo L.
Araújo, Rosélia L.S.
Gimeno, Eduardo Juan
Ortega, Hugo H.
author_sort Sant'Ana, Fabiano J.F.
title Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
title_short Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
title_full Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
title_fullStr Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
title_full_unstemmed Cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
title_sort cytoskeletal proteins in the follicular wall of normal and cystic ovaries of sows
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/86660
work_keys_str_mv AT santanafabianojf cytoskeletalproteinsinthefollicularwallofnormalandcysticovariesofsows
AT reisjuniorjanildol cytoskeletalproteinsinthefollicularwallofnormalandcysticovariesofsows
AT araujoroselials cytoskeletalproteinsinthefollicularwallofnormalandcysticovariesofsows
AT gimenoeduardojuan cytoskeletalproteinsinthefollicularwallofnormalandcysticovariesofsows
AT ortegahugoh cytoskeletalproteinsinthefollicularwallofnormalandcysticovariesofsows
AT santanafabianojf proteinasdocitoesqueletonaparedefoliculardeovariosnormaisecisticosdeporcasmatrizes
AT reisjuniorjanildol proteinasdocitoesqueletonaparedefoliculardeovariosnormaisecisticosdeporcasmatrizes
AT araujoroselials proteinasdocitoesqueletonaparedefoliculardeovariosnormaisecisticosdeporcasmatrizes
AT gimenoeduardojuan proteinasdocitoesqueletonaparedefoliculardeovariosnormaisecisticosdeporcasmatrizes
AT ortegahugoh proteinasdocitoesqueletonaparedefoliculardeovariosnormaisecisticosdeporcasmatrizes
bdutipo_str Repositorios
_version_ 1764820489153282048