The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots

Adhesion of symbiotic bacteria to host plants is an essential early step of the infection process that leads to the beneficial interaction. In the <i>Bradyrhizobium diazoefficiens</i>-soybean symbiosis few molecular determinants of adhesion are known. Here we identified the tight-adhesio...

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Autores principales: Mongiardini, Elías Javier, Parisi, Gustavo Daniel, Quelas, Juan Ignacio, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Exactas
Adhesion
Bradyrhizobium
Infectivity
Integrin
Pili
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86618
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86618
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Adhesion
Bradyrhizobium
Infectivity
Integrin
Pili
spellingShingle Ciencias Exactas
Adhesion
Bradyrhizobium
Infectivity
Integrin
Pili
Mongiardini, Elías Javier
Parisi, Gustavo Daniel
Quelas, Juan Ignacio
Lodeiro, Aníbal Roberto
The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots
topic_facet Ciencias Exactas
Adhesion
Bradyrhizobium
Infectivity
Integrin
Pili
description Adhesion of symbiotic bacteria to host plants is an essential early step of the infection process that leads to the beneficial interaction. In the <i>Bradyrhizobium diazoefficiens</i>-soybean symbiosis few molecular determinants of adhesion are known. Here we identified the tight-adhesion gene products TadGEF in the open-reading frames blr3941-blr3943 of the <i>B. diazoefficiens</i> USDA 110 complete genomic sequence. Predicted structure of TadG indicates a transmembrane domain and two extracytosolic domains, from which the C-terminal has an integrin fold. TadE and TadF are also predicted as bearing transmembrane segments. Mutants in <i>tadG</i> or the small cluster <i>tadGEF</i> were impaired in adhesion to soybean roots, and the root infection was delayed. However, nodule histology was not compromised by the mutations, indicating that these effects were restricted to the earliest contact of the <i>B. diazoefficiens</i> and root surfaces. Knowledge of preinfection determinants is important for development of inoculants that are applied to soybean crops worldwide.
format Articulo
Articulo
author Mongiardini, Elías Javier
Parisi, Gustavo Daniel
Quelas, Juan Ignacio
Lodeiro, Aníbal Roberto
author_facet Mongiardini, Elías Javier
Parisi, Gustavo Daniel
Quelas, Juan Ignacio
Lodeiro, Aníbal Roberto
author_sort Mongiardini, Elías Javier
title The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots
title_short The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots
title_full The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots
title_fullStr The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots
title_full_unstemmed The tight-adhesion proteins TadGEF of <i>Bradyrhizobium diazoefficiens</i> USDA 110 are involved in cell adhesion and infectivity on soybean roots
title_sort tight-adhesion proteins tadgef of <i>bradyrhizobium diazoefficiens</i> usda 110 are involved in cell adhesion and infectivity on soybean roots
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86618
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