SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to imm...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2016
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86612 |
| Aporte de: |
| id |
I19-R120-10915-86612 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Surface Plasmon Resonance assays adsorption |
| spellingShingle |
Ciencias Exactas Surface Plasmon Resonance assays adsorption Daza Millone, María Antonieta Ramírez, Eduardo Alejandro Chain, Cecilia Yamil Crivaro, Andrea Natalia Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo Horacio Cocco, Mauro D. Pedano, María L. Fainstein, Alejandro Montoya, Jorgelina Vela, María Elena Salvarezza, Roberto Carlos SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System |
| topic_facet |
Ciencias Exactas Surface Plasmon Resonance assays adsorption |
| description |
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to immobilize a protein derivative of the analyte, which usually involves the appearance of nonspecific protein binding which limits the detection range of the assay. In this work we present results of a poly-L-lysine (Au-MUA-PLL) based sensor platform for quantitative determination of 2,4-dinitrophenol as model system for small molecular weight substances detection. The prepared sensor chip was characterized by means of Atomic Force Microscopy, Surface Plasmon Resonance, and Surface Enhanced Raman Spectroscopy. Experiments verified the absence of nonspecific protein adsorption to Au-MUA-PLL surfaces and the improvement of the competitive inhibition assays performance in comparison with single and mixed thiol self-assembled monolayers. The possibility of directly immobilizing 2,4-dinitrophenol to the poly-L-lysine containing platforms leads to an improvement in the detection of the soluble analyte by the competitive inhibition assay avoiding undesirable nonspecific protein adsorption. Therefore, Au-MUA-PLL surfaces constitute a suitable alternative for quantitative detection of small molecules when nonspecific adsorption cannot be avoided. |
| format |
Articulo Articulo |
| author |
Daza Millone, María Antonieta Ramírez, Eduardo Alejandro Chain, Cecilia Yamil Crivaro, Andrea Natalia Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo Horacio Cocco, Mauro D. Pedano, María L. Fainstein, Alejandro Montoya, Jorgelina Vela, María Elena Salvarezza, Roberto Carlos |
| author_facet |
Daza Millone, María Antonieta Ramírez, Eduardo Alejandro Chain, Cecilia Yamil Crivaro, Andrea Natalia Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo Horacio Cocco, Mauro D. Pedano, María L. Fainstein, Alejandro Montoya, Jorgelina Vela, María Elena Salvarezza, Roberto Carlos |
| author_sort |
Daza Millone, María Antonieta |
| title |
SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System |
| title_short |
SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System |
| title_full |
SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System |
| title_fullStr |
SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System |
| title_full_unstemmed |
SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System |
| title_sort |
spr biosensing mua/poly-l-lysine platform for the detection of 2,4-dinitrophenol as small molecule model system |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/86612 |
| work_keys_str_mv |
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Repositorios |
| _version_ |
1764820489849536514 |