Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012

Background: As has occurred in many regions worldwide, in 2012 the incidence of pertussis increased in Perú. This epidemiologic situation has been associated with a waning vaccine-induced immunity and the adaptation of Bordetella pertussis to vaccine-induced immunity along with improved diagnostic m...

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Autores principales: Bailon, H., León Janampa, N., Padilla, C., Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Exactas
Bordetella pertussis
Genotype
Pertussis
Perú
Pulsed-field gel electrophoresis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86498
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86498
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella pertussis
Genotype
Pertussis
Perú
Pulsed-field gel electrophoresis
spellingShingle Ciencias Exactas
Bordetella pertussis
Genotype
Pertussis
Perú
Pulsed-field gel electrophoresis
Bailon, H.
León Janampa, N.
Padilla, C.
Hozbor, Daniela Flavia
Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012
topic_facet Ciencias Exactas
Bordetella pertussis
Genotype
Pertussis
Perú
Pulsed-field gel electrophoresis
description Background: As has occurred in many regions worldwide, in 2012 the incidence of pertussis increased in Perú. This epidemiologic situation has been associated with a waning vaccine-induced immunity and the adaptation of Bordetella pertussis to vaccine-induced immunity along with improved diagnostic methods. Methods: The study comprised a total of 840 pertussis-suspected cases reported in Perú during 2012. We summarize here the distribution of pertussis cases according to age and immunization status along with the immunization-coverage rate. Laboratory diagnosis was performed by culture test and real-time polymerase-chain reaction (PCR). B. pertussis bacteria recovered from infected patients were characterized by pulsed-field gel electrophoresis (PFGE), and the DNA sequencing of the pertussis-toxin (promoter and subunit A), pertactin, and fimbriae (fim2 and fim3) genes. Results: From the total pertussis-suspected cases, 191 (22.7 %) infections were confirmed by real-time PCR and 18 through cultivation of B. pertussis (2.1 %), while one infection of B. parapertussis (0.11 %) was also detected by culture. Pertussis was significantly higher in patients that had had 0-3 vaccine doses (pentavalent vaccine alone) than in those who had had 4-5 vaccine doses (pentavalent plus DwPT boosters) at 94.3 vs. 5.7 %, respectively (p < 0.00001). The relative risk (RR) for patients with 4-5 doses compared to those with fewer than 4 doses or no dose was 0.23 (95 % Confidence Interval: 0.11-0.44), while the vaccine effectiveness was 77 % and coverage 50.5 %. Genetic analysis of B. pertussis isolates from different Peruvian regions detected two clonal groups as identified by PFGE. Those two groups corresponded to the B. pertussis genotypes emerging worldwide ptxP3-ptxA1-prn2 or 9-fim3-1 and ptxP3-ptxA1-prn2 or 9-fim3-2. Conclusions: Two emerging B. pertussis genotypes similar to isolates involved in worldwide epidemics were detected in Perú. Low vaccine coverage (<50 %) and genetic divergence between the vaccine-producing strain and the local isolates could contribute to this pertussal epidemic.
format Articulo
Articulo
author Bailon, H.
León Janampa, N.
Padilla, C.
Hozbor, Daniela Flavia
author_facet Bailon, H.
León Janampa, N.
Padilla, C.
Hozbor, Daniela Flavia
author_sort Bailon, H.
title Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012
title_short Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012
title_full Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012
title_fullStr Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012
title_full_unstemmed Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012
title_sort increase in pertussis cases along with high prevalence of two emerging genotypes of bordetella pertussis in perú, 2012
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86498
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AT padillac increaseinpertussiscasesalongwithhighprevalenceoftwoemerginggenotypesofbordetellapertussisinperu2012
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