Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>

Biofilm formation is important for infection by many pathogens. <i>Bordetella bronchiseptica</i> causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formatio...

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Autores principales: Ambrosis, Nicolás Martín, Boyd, Chelsea D., O'Toole, George A., Fernández, Julieta, Sisti, Federico
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Exactas
Bordetella bronchiseptica
respiratory tract infections
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86497
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86497
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella bronchiseptica
respiratory tract infections
spellingShingle Ciencias Exactas
Bordetella bronchiseptica
respiratory tract infections
Ambrosis, Nicolás Martín
Boyd, Chelsea D.
O'Toole, George A.
Fernández, Julieta
Sisti, Federico
Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
topic_facet Ciencias Exactas
Bordetella bronchiseptica
respiratory tract infections
description Biofilm formation is important for infection by many pathogens. <i>Bordetella bronchiseptica</i> causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in <i>B. bronchiseptica</i>. In the present work, based on their previously reported function in <i>Pseudomonas fluorescens</i>, we identified three genes in the <i>B. bronchiseptica</i> genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. <i>In vitro</i> and <i>in vivo</i> studies showed that the protease LapG of <i>B. bronchiseptica</i> cleaves the N-terminal domain of BrtA, as well as the LapA protein of <i>P. fluorescens</i>, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a <i>B. bronchiseptica</i> strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by <i>B. bronchiseptica</i>.
format Articulo
Articulo
author Ambrosis, Nicolás Martín
Boyd, Chelsea D.
O'Toole, George A.
Fernández, Julieta
Sisti, Federico
author_facet Ambrosis, Nicolás Martín
Boyd, Chelsea D.
O'Toole, George A.
Fernández, Julieta
Sisti, Federico
author_sort Ambrosis, Nicolás Martín
title Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
title_short Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
title_full Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
title_fullStr Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
title_full_unstemmed Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i>
title_sort homologs of the lapd-lapg c-di-gmp effector system control biofilm formation by <i>bordetella bronchiseptica</i>
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86497
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bdutipo_str Repositorios
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