A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i>
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiol...
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2016
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86492 |
| Aporte de: |
| id |
I19-R120-10915-86492 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Sinorhizobium meliloti |
| spellingShingle |
Ciencias Exactas Sinorhizobium meliloti Draghi, Walter Omar Del Papa, María Florencia Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, Mauricio Javier Lagares, A. Salas, María Eugenia López, José Luis Albicoro, Francisco Javier Nilsson, Juliet Fernanda Torres Tejerizo, Gonzalo Arturo Luna, María Flavia Pistorio, Mariano Boiardi, José Luis Pühler, A. Weidner, S. Niehaus, K. Lagares, Antonio A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| topic_facet |
Ciencias Exactas Sinorhizobium meliloti |
| description |
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium <i>Sinorhizobium meliloti</i> growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that <i>S. meliloti</i> stopped growing at ca. pH 6.0-6.1. Under such stress the rhizobia increased the O<SUB>2</SUB> consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia. |
| format |
Articulo Articulo |
| author |
Draghi, Walter Omar Del Papa, María Florencia Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, Mauricio Javier Lagares, A. Salas, María Eugenia López, José Luis Albicoro, Francisco Javier Nilsson, Juliet Fernanda Torres Tejerizo, Gonzalo Arturo Luna, María Flavia Pistorio, Mariano Boiardi, José Luis Pühler, A. Weidner, S. Niehaus, K. Lagares, Antonio |
| author_facet |
Draghi, Walter Omar Del Papa, María Florencia Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, Mauricio Javier Lagares, A. Salas, María Eugenia López, José Luis Albicoro, Francisco Javier Nilsson, Juliet Fernanda Torres Tejerizo, Gonzalo Arturo Luna, María Flavia Pistorio, Mariano Boiardi, José Luis Pühler, A. Weidner, S. Niehaus, K. Lagares, Antonio |
| author_sort |
Draghi, Walter Omar |
| title |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_short |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_full |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_fullStr |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_full_unstemmed |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_sort |
consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>sinorhizobium meliloti</i> |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/86492 |
| work_keys_str_mv |
AT draghiwalteromar aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT delpapamariaflorencia aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT hellwegc aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT wattsa aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT watttf aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT barscha aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lozanomauriciojavier aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lagaresa aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT salasmariaeugenia aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lopezjoseluis aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT albicorofranciscojavier aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT nilssonjulietfernanda aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT torrestejerizogonzaloarturo aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lunamariaflavia aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT pistoriomariano aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT boiardijoseluis aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT puhlera aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT weidners aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT niehausk aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lagaresantonio aconsolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT draghiwalteromar consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT delpapamariaflorencia consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT hellwegc consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT wattsa consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT watttf consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT barscha consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lozanomauriciojavier consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lagaresa consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT salasmariaeugenia consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lopezjoseluis consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT albicorofranciscojavier consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT nilssonjulietfernanda consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT torrestejerizogonzaloarturo consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lunamariaflavia consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT pistoriomariano consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT boiardijoseluis consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT puhlera consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT weidners consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT niehausk consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii AT lagaresantonio consolidatedanalysisofthephysiologicandmolecularresponsesinducedunderacidstressinthelegumesymbiontmodelsoilbacteriumisinorhizobiummelilotii |
| bdutipo_str |
Repositorios |
| _version_ |
1764820489734193156 |