SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>

Senescence involves increased expression of proteases, which may participate in nitrogen recycling or cellular signalling. 2D zymograms detected two protein species with increased proteolytic activity in senescing leaves of <i>Arabidopsis thaliana</i>. A proteomic analysis revealed that...

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Autores principales: Martínez, Dana Ethel, Borniego, María Lucía, Battchikova, Natalia, Aro, Eva Mari, Tyystjärvi, Esa, Guiamet, Juan José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Naturales
Ciencias Agrarias
At3g14067
Inflorescence
Protease activity
Reproductive development
Senescence
Subtilisin protease
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86488
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86488
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Ciencias Agrarias
At3g14067
Inflorescence
Protease activity
Reproductive development
Senescence
Subtilisin protease
spellingShingle Ciencias Naturales
Ciencias Agrarias
At3g14067
Inflorescence
Protease activity
Reproductive development
Senescence
Subtilisin protease
Martínez, Dana Ethel
Borniego, María Lucía
Battchikova, Natalia
Aro, Eva Mari
Tyystjärvi, Esa
Guiamet, Juan José
SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>
topic_facet Ciencias Naturales
Ciencias Agrarias
At3g14067
Inflorescence
Protease activity
Reproductive development
Senescence
Subtilisin protease
description Senescence involves increased expression of proteases, which may participate in nitrogen recycling or cellular signalling. 2D zymograms detected two protein species with increased proteolytic activity in senescing leaves of <i>Arabidopsis thaliana</i>. A proteomic analysis revealed that both protein species correspond to a subtilisin protease encoded by At3g14067, termed Senescence-Associated Subtilisin Protease (SASP). <i>SASP</i> mRNA levels and enzyme activity increase during leaf senescence in leaves senescing during both the vegetative or the reproductive phase of the plant life cycle, but this increase is more pronounced in reproductive plants. <i>SASP</i> is expressed in all above-ground organs, but not in roots. Putative AtSASP orthologues were identified in dicot and monocot crop species. A phylogenetic analysis shows AtSASP and its putative orthologues clustering in one discrete group of subtilisin proteases in which no other <i>Arabidospsis</i> subtilisin protease is present. Phenotypic analysis of two knockout lines for <i>SASP</i> showed that mutant plants develop more inflorescence branches during reproductive development. Both <i>AtSASP</i>and its putative rice orthologue (<i>OsSASP</i>) were constitutively expressed in <i>sasp-1</i> to complement the mutant phenotype. At maturity, <i>sasp-1</i> plants produced 25% more inflorescence branches and siliques than either the wild-type or the rescued lines. These differences were mostly due to an increased number of second and third order branches. The increased number of siliques was compensated for by a small decrease (5.0%) in seed size. <i>SASP</i> downregulates branching and silique production during monocarpic senescence, and its function is at least partially conserved between <i>Arabidospsis</i> and rice.
format Articulo
Articulo
author Martínez, Dana Ethel
Borniego, María Lucía
Battchikova, Natalia
Aro, Eva Mari
Tyystjärvi, Esa
Guiamet, Juan José
author_facet Martínez, Dana Ethel
Borniego, María Lucía
Battchikova, Natalia
Aro, Eva Mari
Tyystjärvi, Esa
Guiamet, Juan José
author_sort Martínez, Dana Ethel
title SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>
title_short SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>
title_full SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>
title_fullStr SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>
title_full_unstemmed SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in <i>Arabidopsis</i>
title_sort sasp, a senescence-associated subtilisin protease, is involved in reproductive development and determination of silique number in <i>arabidopsis</i>
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/86488
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