Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions

The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin...

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Autores principales: Smaldini, Paola Lorena, Orsini Delgado, María Lucía, Fossati, Carlos Alberto, Docena, Guillermo Horacio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
Oral immunotherapy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86454
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86454
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Oral immunotherapy
spellingShingle Ciencias Exactas
Oral immunotherapy
Smaldini, Paola Lorena
Orsini Delgado, María Lucía
Fossati, Carlos Alberto
Docena, Guillermo Horacio
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
topic_facet Ciencias Exactas
Oral immunotherapy
description The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.
format Articulo
Articulo
author Smaldini, Paola Lorena
Orsini Delgado, María Lucía
Fossati, Carlos Alberto
Docena, Guillermo Horacio
author_facet Smaldini, Paola Lorena
Orsini Delgado, María Lucía
Fossati, Carlos Alberto
Docena, Guillermo Horacio
author_sort Smaldini, Paola Lorena
title Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_short Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_full Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_fullStr Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_full_unstemmed Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_sort orally-induced intestinal cd4<sup>+</sup> cd25<sup>+</sup> foxp3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/86454
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