Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase

<i>Bordetella bronchiseptica</i> is a Gram-negative bacterium responsible for respiratory diseases in many mammalian hosts, including humans. This pathogen has been shown as able to persist inside the host cells, even in the phagosomes that are acidified to pH 4.5-5.0 after bacterial inf...

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Detalles Bibliográficos
Autores principales: Fingermann, Matías, Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
Acid-tolerance response
Bordetella bronchiseptica
Proteomics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86452
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86452
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Acid-tolerance response
Bordetella bronchiseptica
Proteomics
spellingShingle Ciencias Exactas
Acid-tolerance response
Bordetella bronchiseptica
Proteomics
Fingermann, Matías
Hozbor, Daniela Flavia
Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase
topic_facet Ciencias Exactas
Acid-tolerance response
Bordetella bronchiseptica
Proteomics
description <i>Bordetella bronchiseptica</i> is a Gram-negative bacterium responsible for respiratory diseases in many mammalian hosts, including humans. This pathogen has been shown as able to persist inside the host cells, even in the phagosomes that are acidified to pH 4.5-5.0 after bacterial infection. Here we evaluated the resistance of <i>B. bronchiseptica</i> to survive under acidic conditions. In particular we analyzed the bacterial capacity to develop the mechanism known as acid tolerance response (ATR). Our studies were mainly focused on the avirulent phase of the bacteria since this phenotypic phase was reported to be more resistant to environmental stress conditions than the virulent phase. Results from <i>B. bronchiseptica</i> in virulent phase were also included for comparison purposes. In fact, for <i>B. bronchiseptica</i> 9.73 bacteria in virulent phase we observed that the viability of bacteria does not decrease significantly when grown at pH as low as 4.5, but it is affected when the pH of the medium was equal to or less than 4.0. After acid-adaptation at pH 5.5 for several hours, the survival rate of <i>B. bronchiseptica</i> 9.73 at lethal pH 4.0 for 6. h was increased. Interestingly, the avirulent phase mediated by the two-component BvgAS system conferred further resistance to lethal acid challenge and a marked increase in the magnitude of the expressed ATR. The ATR for this avirulent phase seems to be associated with changes in LPS and surface protein profiles. 2D-gel electrophoresis revealed at least 25 polypeptides differentially expressed, 17 of which were only expressed or over-expressed under acid conditions. Using MALDI-TOF mass spectrometry, 10 of these differentially expressed polypeptides were identified.
format Articulo
Articulo
author Fingermann, Matías
Hozbor, Daniela Flavia
author_facet Fingermann, Matías
Hozbor, Daniela Flavia
author_sort Fingermann, Matías
title Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase
title_short Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase
title_full Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase
title_fullStr Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase
title_full_unstemmed Acid tolerance response of <i>Bordetella bronchiseptica</i> in avirulent phase
title_sort acid tolerance response of <i>bordetella bronchiseptica</i> in avirulent phase
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/86452
work_keys_str_mv AT fingermannmatias acidtoleranceresponseofibordetellabronchisepticaiinavirulentphase
AT hozbordanielaflavia acidtoleranceresponseofibordetellabronchisepticaiinavirulentphase
bdutipo_str Repositorios
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