Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>

NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of <i>Leptospira</i> spp. to induce NETosis using human <i>ex vivo</i> and murine <i>in vivo&l...

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Autores principales: Scharrig, Emilia, Carestia, Agostina, Ferrer, María Florencia, Cedola, Maia Tatiana, Prêtre, Gabriela, Drut, Ricardo, Picardeau, Mathieu, Schattner, Mirta, Gómez, Ricardo Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
Leptospirosis
Leptospira
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86367
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86367
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Leptospirosis
Leptospira
spellingShingle Ciencias Exactas
Leptospirosis
Leptospira
Scharrig, Emilia
Carestia, Agostina
Ferrer, María Florencia
Cedola, Maia Tatiana
Prêtre, Gabriela
Drut, Ricardo
Picardeau, Mathieu
Schattner, Mirta
Gómez, Ricardo Martín
Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>
topic_facet Ciencias Exactas
Leptospirosis
Leptospira
description NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of <i>Leptospira</i> spp. to induce NETosis using human <i>ex vivo</i> and murine <i>in vivo</i> models. Microscopy and fluorometric studies showed that incubation of human neutrophils with <i>Leptospira interrogans</i> serovar Copenhageni strain Fiocruz L1-130 (LIC) resulted in the release of DNA extracellular traps (NETs). The bacteria number, pathogenicity and viability were relevant factors for induction of NETs, but bacteria motility was not. Entrapment of LIC in the NETs resulted in LIC death; however, pathogenic but not saprophytic <i>Leptospira</i> sp. exerted nuclease activity and degraded DNA. Mice infected with LIC showed circulating NETs after 2 days post-infection (dpi). Depletion of neutrophils with mAb1A8 significantly reduced the amount of intravascular NETs in LIC-infected mice, increasing bacteremia at 3 dpi. Although there was a low bacterial burden, scarce neutrophils and an absence of inflammation in the early stages of infection in the kidney and liver, at the beginning of the leptospiruric phase, the bacterial burden was significantly higher in kidneys of neutrophil-depleted-mice compared to non-depleted and infected mice. Surprisingly, interstitial nephritis was of similar intensity in both groups of infected mice. Taken together, these data suggest that LIC triggers NETs, and that the intravascular formation of these DNA traps appears to be critical not only to prevent early leptospiral dissemination but also to preclude further bacterial burden.
format Articulo
Articulo
author Scharrig, Emilia
Carestia, Agostina
Ferrer, María Florencia
Cedola, Maia Tatiana
Prêtre, Gabriela
Drut, Ricardo
Picardeau, Mathieu
Schattner, Mirta
Gómez, Ricardo Martín
author_facet Scharrig, Emilia
Carestia, Agostina
Ferrer, María Florencia
Cedola, Maia Tatiana
Prêtre, Gabriela
Drut, Ricardo
Picardeau, Mathieu
Schattner, Mirta
Gómez, Ricardo Martín
author_sort Scharrig, Emilia
title Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>
title_short Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>
title_full Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>
title_fullStr Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>
title_full_unstemmed Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>
title_sort neutrophil extracellular traps are involved in the innate immune response to infection with <i>leptospira</i>
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/86367
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